Kinetic constants for substrate binding for purified wild-type and Arg127Cys mutant γ-GCSH protein
. | Wild-type GCSH . | Arg127Cys . |
|---|---|---|
| Vmax, μmol/mg/h | 531.75 ± 13.77 | 26.48 ± 0.97 |
| Km glu, mM | 7.16 ± 0.62 | 0.63 ± 0.17 |
| Km aba, mM | 2.39 ± 0.63 | 0.25 ± 0.05 |
| Ki GSH, mM | 0.45 ± 0.04 | > 1000 |
. | Wild-type GCSH . | Arg127Cys . |
|---|---|---|
| Vmax, μmol/mg/h | 531.75 ± 13.77 | 26.48 ± 0.97 |
| Km glu, mM | 7.16 ± 0.62 | 0.63 ± 0.17 |
| Km aba, mM | 2.39 ± 0.63 | 0.25 ± 0.05 |
| Ki GSH, mM | 0.45 ± 0.04 | > 1000 |
Error represents the SEM calculated from 3 separate experiments