Liver histopathology before and after BMT
. | Naive . | Syn day 35 . | Allo day 7 . | Allo day 35 . |
|---|---|---|---|---|
| Portal triads | ||||
| Portal tract expansion | 0.3±0.6 | 0.3±0.6 | 0±0 | 1.7±0.7† |
| Neutrophil infiltrate | 0±0 | 0±0 | 0±0 | 1.3±0.6*† |
| Mononuclear cell infiltrate | 0±0 | 0.3±0.6 | 0±0 | 1.3±0.6† |
| Bile ducts/ductules | ||||
| Mononuclear cell infiltrates | 0±0 | 0±0 | 0±0 | 1±0*† |
| Nuclear pleomorphism | 0.3±0.6 | 0±0 | 0±0 | 1±0*† |
| Cytoplasmic eosinophilia | 0±0 | 0±0 | 0±0 | 1±0*† |
| Nuclear multilayering | 0±0 | 0±0 | 0±0 | 0.7±0.6 |
| Pyknotic duct cells | 0±0 | 0±0 | 0±0 | 1±0*† |
| Intraluminal epithelial cells | 0±0 | 0±0 | 0±0 | 0.3±0.6 |
| Vessels | ||||
| Endotheliatis | 0±0 | 0±0 | 0±0 | 1.7±0.6*† |
| Mononuclear cells around CV | 0±0 | 0±0 | 0±0 | 0.7±0.6 |
| Hepatocytes | ||||
| Confluent necrosis | 0±0 | 0±0 | 0.3±0.6 | 0±0 |
| Acidophilic bodies | 0.3±0.6 | 0.7±0.6 | 1.3±0.6 | 1.7±1.2 |
| Mitotic figures | 0±0 | 0±0 | 0±0 | 1±0*† |
| Neutrophil accumulations | 0.7±0.6 | 0±0 | 0±0 | 1±0*† |
| Macrophage aggregates | 0.3±0.6 | 0±0 | 0.3±0.6 | 1±0* |
| Total score | 2.0±2.6 | 1.3±1.5 | 2.0±1.7 | 16.3±2.9*† |
. | Naive . | Syn day 35 . | Allo day 7 . | Allo day 35 . |
|---|---|---|---|---|
| Portal triads | ||||
| Portal tract expansion | 0.3±0.6 | 0.3±0.6 | 0±0 | 1.7±0.7† |
| Neutrophil infiltrate | 0±0 | 0±0 | 0±0 | 1.3±0.6*† |
| Mononuclear cell infiltrate | 0±0 | 0.3±0.6 | 0±0 | 1.3±0.6† |
| Bile ducts/ductules | ||||
| Mononuclear cell infiltrates | 0±0 | 0±0 | 0±0 | 1±0*† |
| Nuclear pleomorphism | 0.3±0.6 | 0±0 | 0±0 | 1±0*† |
| Cytoplasmic eosinophilia | 0±0 | 0±0 | 0±0 | 1±0*† |
| Nuclear multilayering | 0±0 | 0±0 | 0±0 | 0.7±0.6 |
| Pyknotic duct cells | 0±0 | 0±0 | 0±0 | 1±0*† |
| Intraluminal epithelial cells | 0±0 | 0±0 | 0±0 | 0.3±0.6 |
| Vessels | ||||
| Endotheliatis | 0±0 | 0±0 | 0±0 | 1.7±0.6*† |
| Mononuclear cells around CV | 0±0 | 0±0 | 0±0 | 0.7±0.6 |
| Hepatocytes | ||||
| Confluent necrosis | 0±0 | 0±0 | 0.3±0.6 | 0±0 |
| Acidophilic bodies | 0.3±0.6 | 0.7±0.6 | 1.3±0.6 | 1.7±1.2 |
| Mitotic figures | 0±0 | 0±0 | 0±0 | 1±0*† |
| Neutrophil accumulations | 0.7±0.6 | 0±0 | 0±0 | 1±0*† |
| Macrophage aggregates | 0.3±0.6 | 0±0 | 0.3±0.6 | 1±0* |
| Total score | 2.0±2.6 | 1.3±1.5 | 2.0±1.7 | 16.3±2.9*† |
Parameters of liver histopathology from naive mice or BMT recipients at either day 7 or day 35 after BMT were scored from 0 to 4 depending on extent and severity of injury as described in “Materials and methods.” Results represent means ± standard deviations. The Wilcoxon rank-sum test was used for the statistical analysis. Most parameters of injury are significantly worse in allo day 35 group versus syn day 35 group or versus allo day 7 group
All values were 0±0 for syn day 7 group
Syn indicates syngeneic; allo, allogeneic; and CV, central vein
P = .05
P = .05