Proteasomal cleavage prediction of HA-3 alleles using the NetChop C-term 2.0 and the PAProc human type III algorithms
. | . | NetChop C-term 2.0 . | . | . | . | PAProc human type III . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Position . | Amino acid . | Thr . | . | Met . | . | Thr . | Met . | ||||
| 1 | Asp | — | 0.154 | — | 0.154 | +++ | +++ | ||||
| 2 | Ala | — | 0.038 | — | 0.032 | - | - | ||||
| 3 | Val | — | 0.032 | — | 0.056 | - | - | ||||
| 4 | Leu | S | 1.000 | S | 1.000 | +++ | +++ | ||||
| 5 | Gln | — | 0.003 | — | 0.002 | - | - | ||||
| 6 | Arg | S | 0.982 | S | 0.993 | ++ | ++ | ||||
| 7 | Asp | — | 0.281 | — | 0.266 | - | - | ||||
| 8 | Leu | — | 0.019 | — | 0.004 | - | - | ||||
| 9 | Val | — | 0.284 | — | 0.302 | - | - | ||||
| 10 | Thr/Met | — | 0.008 | S | 0.560 | - | +++ | ||||
| 11 | Glu | — | 0.001 | — | 0.001 | - | - | ||||
| 12 | Pro | — | 0.036 | — | 0.024 | + | - | ||||
| 13 | Gly | — | 0.206 | — | 0.051 | - | - | ||||
| 14 | Thr | — | 0.093 | — | 0.033 | - | - | ||||
| 15 | Ala | S | 0.999 | S | 0.994 | - | - | ||||
| 16 | Gln | S | 0.688 | — | 0.381 | +++ | +++ | ||||
| 17 | Tyr | S | 1.000 | S | 1.000 | +++ | +++ | ||||
| 18 | Ser | — | 0.319 | S | 0.663 | + | + | ||||
| 19 | Ser | — | 0.458 | — | 0.458 | + | + | ||||
| 20 | Gly | — | 0.057 | — | 0.057 | - | - | ||||
| 21 | Gly | — | 0.089 | — | 0.089 | + | + | ||||
| 22 | Glu | — | 0.000 | — | 0.000 | - | - | ||||
| 23 | Leu | S | 0.907 | S | 0.907 | - | - | ||||
| 24 | Gly | — | 0.004 | — | 0.004 | + | + | ||||
| 25 | Gly | — | 0.026 | — | 0.026 | - | - | ||||
. | . | NetChop C-term 2.0 . | . | . | . | PAProc human type III . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Position . | Amino acid . | Thr . | . | Met . | . | Thr . | Met . | ||||
| 1 | Asp | — | 0.154 | — | 0.154 | +++ | +++ | ||||
| 2 | Ala | — | 0.038 | — | 0.032 | - | - | ||||
| 3 | Val | — | 0.032 | — | 0.056 | - | - | ||||
| 4 | Leu | S | 1.000 | S | 1.000 | +++ | +++ | ||||
| 5 | Gln | — | 0.003 | — | 0.002 | - | - | ||||
| 6 | Arg | S | 0.982 | S | 0.993 | ++ | ++ | ||||
| 7 | Asp | — | 0.281 | — | 0.266 | - | - | ||||
| 8 | Leu | — | 0.019 | — | 0.004 | - | - | ||||
| 9 | Val | — | 0.284 | — | 0.302 | - | - | ||||
| 10 | Thr/Met | — | 0.008 | S | 0.560 | - | +++ | ||||
| 11 | Glu | — | 0.001 | — | 0.001 | - | - | ||||
| 12 | Pro | — | 0.036 | — | 0.024 | + | - | ||||
| 13 | Gly | — | 0.206 | — | 0.051 | - | - | ||||
| 14 | Thr | — | 0.093 | — | 0.033 | - | - | ||||
| 15 | Ala | S | 0.999 | S | 0.994 | - | - | ||||
| 16 | Gln | S | 0.688 | — | 0.381 | +++ | +++ | ||||
| 17 | Tyr | S | 1.000 | S | 1.000 | +++ | +++ | ||||
| 18 | Ser | — | 0.319 | S | 0.663 | + | + | ||||
| 19 | Ser | — | 0.458 | — | 0.458 | + | + | ||||
| 20 | Gly | — | 0.057 | — | 0.057 | - | - | ||||
| 21 | Gly | — | 0.089 | — | 0.089 | + | + | ||||
| 22 | Glu | — | 0.000 | — | 0.000 | - | - | ||||
| 23 | Leu | S | 0.907 | S | 0.907 | - | - | ||||
| 24 | Gly | — | 0.004 | — | 0.004 | + | + | ||||
| 25 | Gly | — | 0.026 | — | 0.026 | - | - | ||||
NetChop results are listed as follows: S indicates a cleavage position behind the indicated amino acid and the values list the probability of being cleaved for each position; and — indicates no cleavage predicted; and PAProc results are listed as follows: - indicates no cleavage behind this position; and +, ++, +++, cleavage behind this position where the number of + indicate the predicted cleavage strength
Shading indicates the HA-3 CTL epitope