Primers for quantification of Hepc, TfR1, TfR2, IREG1, DMT1-IRE, DMT1-nonIRE, and Cp transcripts using the LightCycler RT-PCR assay
Primer . | Sequence . | Orientation . |
|---|---|---|
| Hepc | ||
| Hepc-501 | 5′-CTG CAA CCC CAG GAC AGA G-3′ | Sense |
| Hepc-301 | 5′-GGA ATA AAT AAG GAA GGG AGG GG-3′ | Antisense |
| TfR1 | ||
| TfR1-502 | 5′-TAT AGA AGG TTT GGG GGC TGT G-3′ | Sense |
| TfR1-302 | 5′-GAG ACC CTA TGA ACT TTT CCC TAG-3′ | Antisense |
| TfR2 | ||
| TfR2-501 | 5′-GAT TCA GGG TCA GGG AGG TG-3′ | Sense |
| TfR2-301 | 5′-GAA GGG GCT GTG ATT GAA GG-3′ | Antisense |
| IREG1 | ||
| IREG1-501 | 5′-CTT CAG CCT GGC AAG TTA CAT G-3′ | Sense |
| IREG1-301 | 5′-TTC TCA AAG GCA TTT GAA AGG G-3′ | Antisense |
| DMT1-IRE | ||
| DMT-IRE-502 | 5′-CTT CAT ATC TGC CTC TTC CCC-3′ | Sense |
| DMT-IRE-301 | 5′-AAA TCT GAG ACT GAC TGG ACC C-3′ | Antisense |
| DMT-nonIRE | ||
| DMT-nIRE-501 | 5′-TGG TGT GAT CTC AGC TCA CTG-3′ | Sense |
| DMT-nIRE-301 | 5′-GGC CAG CAG ATT ACT TGA GC-3′ | Antisense |
| Cp | ||
| Cp-501 | 5′-ATG GGA ATG GGC AAT GAA ATA G-3′ | Sense |
| Cp-301 | 5′-GCA TGA ATG TGG TCG GTC AC-3′ | Antisense |
Primer . | Sequence . | Orientation . |
|---|---|---|
| Hepc | ||
| Hepc-501 | 5′-CTG CAA CCC CAG GAC AGA G-3′ | Sense |
| Hepc-301 | 5′-GGA ATA AAT AAG GAA GGG AGG GG-3′ | Antisense |
| TfR1 | ||
| TfR1-502 | 5′-TAT AGA AGG TTT GGG GGC TGT G-3′ | Sense |
| TfR1-302 | 5′-GAG ACC CTA TGA ACT TTT CCC TAG-3′ | Antisense |
| TfR2 | ||
| TfR2-501 | 5′-GAT TCA GGG TCA GGG AGG TG-3′ | Sense |
| TfR2-301 | 5′-GAA GGG GCT GTG ATT GAA GG-3′ | Antisense |
| IREG1 | ||
| IREG1-501 | 5′-CTT CAG CCT GGC AAG TTA CAT G-3′ | Sense |
| IREG1-301 | 5′-TTC TCA AAG GCA TTT GAA AGG G-3′ | Antisense |
| DMT1-IRE | ||
| DMT-IRE-502 | 5′-CTT CAT ATC TGC CTC TTC CCC-3′ | Sense |
| DMT-IRE-301 | 5′-AAA TCT GAG ACT GAC TGG ACC C-3′ | Antisense |
| DMT-nonIRE | ||
| DMT-nIRE-501 | 5′-TGG TGT GAT CTC AGC TCA CTG-3′ | Sense |
| DMT-nIRE-301 | 5′-GGC CAG CAG ATT ACT TGA GC-3′ | Antisense |
| Cp | ||
| Cp-501 | 5′-ATG GGA ATG GGC AAT GAA ATA G-3′ | Sense |
| Cp-301 | 5′-GCA TGA ATG TGG TCG GTC AC-3′ | Antisense |