Angiotensin II enhances the extracellular release of O2- by human neutrophils
Additions to cells . | O2-, nmol/106 cells × min . |
|---|---|
| None | 0.056 |
| Ang (10) | 0.142 |
| Ang (100) | 0.417 |
| Ang (1000) | 0.781 |
| Ang (100) + PD098059 | 0.056 |
| Ang (1000) + PD098059 | 0.170 |
Additions to cells . | O2-, nmol/106 cells × min . |
|---|---|
| None | 0.056 |
| Ang (10) | 0.142 |
| Ang (100) | 0.417 |
| Ang (1000) | 0.781 |
| Ang (100) + PD098059 | 0.056 |
| Ang (1000) + PD098059 | 0.170 |
Neutrophils (1 × 106 cells/mL) were preincubated alone or with PD098059 (50 μM) for 30 minutes. Reactions were started by the addition of the indicated concentrations (nM) of Ang II (Ang) and were allowed to proceed for 10 minutes. OD550 from cytochrome c was recorded