Immunohistochemical and molecular analysis of 13 cases of DLBCL with late relapse
. | First episode . | . | . | . | . | . | . | Second episode . | . | . | . | . | . | VH gene analysis . | . | . | . | . | . | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | IgH . | . | . | . | . | . | . | IgH . | . | VDJ usage . | . | . | . | . | . | |||||||||||||||
| Case . | bcl-2 . | CD10 . | bcl-6 . | t(14;18) . | CDR2 . | CDR3 . | Interval (mos) . | bcl-2 . | CD10 . | bcl-6 . | t(14;18) PCR . | CDR2 . | CDR3 . | VH . | DH . | JH . | Shared mutations (per 100 bp) . | Ongoing mutations (per 100 bp) . | Conclusion . | |||||||||||||||
| 1 | + | + | + | − | 231 | 80 | 50 | + | + | ND | − | 231 | 80 | V3-07 | D3-3 | JH4a | 11 (6.11) | 2 (0.91) | Clonally related | |||||||||||||||
| 2 | + | + | + | MCR | − | − | 53 | + | + | + | MCR | − | − | NA | NA | NA | NA | NA | Clonally related | |||||||||||||||
| 3 | + | + | + | − | − | 95 | 66 | + | − | + | − | − | 95 | − | − | JH4 | − | − | Clonally related | |||||||||||||||
| 4 | ND | + | + | − | 255 | 92 | 67 | + | + | + | − | 255 | 92 | V3-48 | D3-3 | JH6 | 7 (5.56) + del 21 bp | 20 (8.30) | Clonally related | |||||||||||||||
| 5 | ND | + | +/− | − | − | − | 76 | + | − | + | − | 261 | − | NA | NA | NA | NA | NA | Unresolved | |||||||||||||||
| 6 | − | − | − | − | − | − | 76 | + | − | − | − | − | − | NA | NA | NA | NA | NA | Unresolved | |||||||||||||||
| 7 | − | − | + | − | 258 | − | 81 | + | − | ND | − | − | − | V3-30.3 | − | JH4b | 14 (7.37) | −(0.00) | Clonally related | |||||||||||||||
| 8 | + | − | ND | − | − | 132 | 91 | + | − | + | − | 266 | 132 | V3-21 | D1-1, D4-23 | − | 25 (13.8) | 2 (2.00) | Clonally related | |||||||||||||||
| 9 | + | + | + | − | − | − | 97 | + | + | + | − | − | − | NA | NA | NA | NA | NA | Unresolved | |||||||||||||||
| 10 | ND | ND | ND | − | 258 | 106 | 110 | + | − | − | − | 258 | 106 | V3-30.5 | D4-17 | JH3b | 16 (9.41) | 0 (0.00) | Clonally related | |||||||||||||||
| 11 | + | − | + | MBR | − | 246 | 141 | + | + | + | MBR | − | 246 | V3-48 | D2-2 | JH4a | 18 (11.32) | 0 (0.00) | Clonally related | |||||||||||||||
| 12 | ND | + | − | − | − | 137 | 144 | + | + | + | − | − | 137 | V4 | D2-8, D3-3inv | − | 0 (0.00) | 2 (2.82) | Clonally related | |||||||||||||||
| 13 | ND | − | + | − | 148 | − | 207 | + | − | + | − | 148 | − | V3-20 | D2-2, D3-3 | JH5 | 0 (0.00) | 2 (1.18) | Clonally related | |||||||||||||||
. | First episode . | . | . | . | . | . | . | Second episode . | . | . | . | . | . | VH gene analysis . | . | . | . | . | . | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | IgH . | . | . | . | . | . | . | IgH . | . | VDJ usage . | . | . | . | . | . | |||||||||||||||
| Case . | bcl-2 . | CD10 . | bcl-6 . | t(14;18) . | CDR2 . | CDR3 . | Interval (mos) . | bcl-2 . | CD10 . | bcl-6 . | t(14;18) PCR . | CDR2 . | CDR3 . | VH . | DH . | JH . | Shared mutations (per 100 bp) . | Ongoing mutations (per 100 bp) . | Conclusion . | |||||||||||||||
| 1 | + | + | + | − | 231 | 80 | 50 | + | + | ND | − | 231 | 80 | V3-07 | D3-3 | JH4a | 11 (6.11) | 2 (0.91) | Clonally related | |||||||||||||||
| 2 | + | + | + | MCR | − | − | 53 | + | + | + | MCR | − | − | NA | NA | NA | NA | NA | Clonally related | |||||||||||||||
| 3 | + | + | + | − | − | 95 | 66 | + | − | + | − | − | 95 | − | − | JH4 | − | − | Clonally related | |||||||||||||||
| 4 | ND | + | + | − | 255 | 92 | 67 | + | + | + | − | 255 | 92 | V3-48 | D3-3 | JH6 | 7 (5.56) + del 21 bp | 20 (8.30) | Clonally related | |||||||||||||||
| 5 | ND | + | +/− | − | − | − | 76 | + | − | + | − | 261 | − | NA | NA | NA | NA | NA | Unresolved | |||||||||||||||
| 6 | − | − | − | − | − | − | 76 | + | − | − | − | − | − | NA | NA | NA | NA | NA | Unresolved | |||||||||||||||
| 7 | − | − | + | − | 258 | − | 81 | + | − | ND | − | − | − | V3-30.3 | − | JH4b | 14 (7.37) | −(0.00) | Clonally related | |||||||||||||||
| 8 | + | − | ND | − | − | 132 | 91 | + | − | + | − | 266 | 132 | V3-21 | D1-1, D4-23 | − | 25 (13.8) | 2 (2.00) | Clonally related | |||||||||||||||
| 9 | + | + | + | − | − | − | 97 | + | + | + | − | − | − | NA | NA | NA | NA | NA | Unresolved | |||||||||||||||
| 10 | ND | ND | ND | − | 258 | 106 | 110 | + | − | − | − | 258 | 106 | V3-30.5 | D4-17 | JH3b | 16 (9.41) | 0 (0.00) | Clonally related | |||||||||||||||
| 11 | + | − | + | MBR | − | 246 | 141 | + | + | + | MBR | − | 246 | V3-48 | D2-2 | JH4a | 18 (11.32) | 0 (0.00) | Clonally related | |||||||||||||||
| 12 | ND | + | − | − | − | 137 | 144 | + | + | + | − | − | 137 | V4 | D2-8, D3-3inv | − | 0 (0.00) | 2 (2.82) | Clonally related | |||||||||||||||
| 13 | ND | − | + | − | 148 | − | 207 | + | − | + | − | 148 | − | V3-20 | D2-2, D3-3 | JH5 | 0 (0.00) | 2 (1.18) | Clonally related | |||||||||||||||
ND indicates not done; NA, not applicable; + indicates positive; and − indicates negative.