Table 1. Phenotypic characterization of... | American Society of Hematology

Table 1.

Phenotypic characterization of cells transduced by a lentiviral vector after stimulation with IL-2, IL-7, and IL-15

	IL-2, %			IL-7, %			IL-15, %
Surface marker	LNGFr⁺	LNGFr^-	LNGFr⁺	LNGFr^-	LNGFr⁺	LNGFr^-	LNGFr⁺	LNGFr^-	PBMC, %
CD3	>90	>90	>90	>90	>90	>90	>90	>90	87
CD4	47	47	48	53	36	44	42	17	53
CD8	20	38	26	35	41	35	47	60	32
CD56	8	5	4	9	13	18	96	24	12
CD62L	56	60	54	69	23	45	88	70	33
CD95	83	89	90	93	91	91	99	96	40
CD25	20	6	23	26	52	43	77	78	1
CD16	7	4	5	2	5	7	1	5	5
CD14	0	0	0	0	0	0	0	0	1
CD45RA	50	30	52	40	38	38	11	30	50
CD45RO	26	59	28	46	41	46	92	70	49

	IL-2, %			IL-7, %			IL-15, %
Surface marker	LNGFr⁺	LNGFr^-	LNGFr⁺	LNGFr^-	LNGFr⁺	LNGFr^-	LNGFr⁺	LNGFr^-	PBMC, %
CD3	>90	>90	>90	>90	>90	>90	>90	>90	87
CD4	47	47	48	53	36	44	42	17	53
CD8	20	38	26	35	41	35	47	60	32
CD56	8	5	4	9	13	18	96	24	12
CD62L	56	60	54	69	23	45	88	70	33
CD95	83	89	90	93	91	91	99	96	40
CD25	20	6	23	26	52	43	77	78	1
CD16	7	4	5	2	5	7	1	5	5
CD14	0	0	0	0	0	0	0	0	1
CD45RA	50	30	52	40	38	38	11	30	50
CD45RO	26	59	28	46	41	46	92	70	49

Transduced T cells previously stimulated with IL-2, IL-7, and IL-15 or TCR-triggered were characterized for the expression of lineage and activation markers and for transgene expression by flow cytometry. Unmodified PBMCs from the same donors were stained as controls. Transduced and untransduced cells were analyzed and compared by gating on LNGFr⁺ and LNGFr^- cell populations. A representative experiment is shown.

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