Development of autoreactive CD4+ T cells in the [II–/– → wt] chimeras
Mice . | Stimulation with B6-PC . | Proliferation, cpm . | IFN-γ, pg/mL . | IL-2, pg/mL . |
|---|---|---|---|---|
| wt B6 | ||||
| - | 538 ± 34 | UD | UD | |
| + | 860 ± 164 | UD | UD | |
| [wt → wt] | ||||
| - | 916 ± 24 | UD | UD | |
| + | 780 ± 157 | UD | UD | |
| [wt → II-/-] | ||||
| - | 733 ± 237 | UD | UD | |
| + | 535 ± 181 | UD | UD | |
| [II-/- → wt] | ||||
| - | 949 ± 54 | UD | UD | |
| + | 5640 ± 459 | 698 ± 22 | 64.1 ± 9.5 |
Mice . | Stimulation with B6-PC . | Proliferation, cpm . | IFN-γ, pg/mL . | IL-2, pg/mL . |
|---|---|---|---|---|
| wt B6 | ||||
| - | 538 ± 34 | UD | UD | |
| + | 860 ± 164 | UD | UD | |
| [wt → wt] | ||||
| - | 916 ± 24 | UD | UD | |
| + | 780 ± 157 | UD | UD | |
| [wt → II-/-] | ||||
| - | 733 ± 237 | UD | UD | |
| + | 535 ± 181 | UD | UD | |
| [II-/- → wt] | ||||
| - | 949 ± 54 | UD | UD | |
| + | 5640 ± 459 | 698 ± 22 | 64.1 ± 9.5 |
CD4+ T cells were isolated from spleens (3 spleens/group) 8 weeks after BMT and were stimulated at 2 × 105 cells/well with irradiated (20 Gy) peritoneal cells (1 × 105/well) from B6 mice (B6-PC). After 3 days of culture, supernatants were harvested for cytokine measurements and cell proliferation was determined after pulsing with [3H]thymidine for an additional 16 hours. Data represent mean ± SD. UD indicates undetectable.