Development of multiple organ injury in [II–/– → wt] chimeras
. | Naive wt . | [wt → wt] . | [II-/- → wt] . | [β2m-/- → wt] . |
|---|---|---|---|---|
| Pathology scores | n = 6 | n = 15 | n = 14 | n = 3 |
| Liver | ||||
| Portal tracts | 0.5 ± 0.3 | 0.3 ± 0.2 | 4.9 ± 0.6* | 0.0 ± 0.0 |
| Bile ducts | 0.0 ± 0.0 | 0.0 ± 0.0 | 2.6 ± 0.5* | 0.0 ± 0.0 |
| Vessels | 0.2 ± 0.2 | 0.1 ± 0.1 | 1.4 ± 0.3† | 0.0 ± 0.0 |
| Hepatocytes | 1.2 ± 0.6 | 1.0 ± 0.2 | 3.4 ± 0.4* | 0.0 ± 0.0 |
| Total | 1.8 ± 0.6 | 1.3 ± 0.2 | 12.6 ± 1.5* | 0.0 ± 0.0 |
| Small bowel | ||||
| Architecture | 1.1 ± 0.5 | 1.1 ± 0.3 | 2.6 ± 0.2* | 0.0 ± 0.0 |
| Epithelium | 1.3 ± 0.5 | 1.5 ± 0.4 | 3.1 ± 0.2† | 0.0 ± 0.0 |
| Total | 2.5 ± 1.0 | 2.5 ± 0.6 | 5.8 ± 0.3* | 0.0 ± 0.0 |
| Large bowel | ||||
| Architecture | 1.0 ± 0.0 | 0.5 ± 0.2 | 1.7 ± 0.3† | 0.0 ± 0.0 |
| Epithelium | 1.5 ± 0.5 | 0.7 ± 0.2 | 2.9 ± 0.2* | 0.0 ± 0.0 |
| Total | 2.3 ± 0.8 | 1.2 ± 0.4 | 4.6 ± 0.4* | 0.0 ± 0.0 |
| Skin | 0.0 ± 0.0 | 0.0 ± 0.0 | 1.3 ± 0.2* | 0.0 ± 0.0 |
| Immune reconstitution | n = 3 | n = 4 | n = 4 | ND |
| Thymus, cells × 106 | ||||
| Total thymocytes | 82 ± 5 | 62 ± 13 | 1 ± 2 | ND |
| CD4 SP cells | 11 ± 1 | 8 ± 2 | 0 ± 0 | ND |
| CD8 SP cells | 2 ± 1 | 2 ± 1 | 0 ± 0 | ND |
| Spleen, cells × 106 | ||||
| CD4+ T cells | 21 ± 2 | 18 ± 2 | 8 ± 4† | ND |
| CD8+ T cells | 9 ± 1 | 6 ± 1 | 3 ± 1† | ND |
| B220+ cells | 43 ± 3 | 55 ± 5.8 | 22 ± 8.6† | ND |
. | Naive wt . | [wt → wt] . | [II-/- → wt] . | [β2m-/- → wt] . |
|---|---|---|---|---|
| Pathology scores | n = 6 | n = 15 | n = 14 | n = 3 |
| Liver | ||||
| Portal tracts | 0.5 ± 0.3 | 0.3 ± 0.2 | 4.9 ± 0.6* | 0.0 ± 0.0 |
| Bile ducts | 0.0 ± 0.0 | 0.0 ± 0.0 | 2.6 ± 0.5* | 0.0 ± 0.0 |
| Vessels | 0.2 ± 0.2 | 0.1 ± 0.1 | 1.4 ± 0.3† | 0.0 ± 0.0 |
| Hepatocytes | 1.2 ± 0.6 | 1.0 ± 0.2 | 3.4 ± 0.4* | 0.0 ± 0.0 |
| Total | 1.8 ± 0.6 | 1.3 ± 0.2 | 12.6 ± 1.5* | 0.0 ± 0.0 |
| Small bowel | ||||
| Architecture | 1.1 ± 0.5 | 1.1 ± 0.3 | 2.6 ± 0.2* | 0.0 ± 0.0 |
| Epithelium | 1.3 ± 0.5 | 1.5 ± 0.4 | 3.1 ± 0.2† | 0.0 ± 0.0 |
| Total | 2.5 ± 1.0 | 2.5 ± 0.6 | 5.8 ± 0.3* | 0.0 ± 0.0 |
| Large bowel | ||||
| Architecture | 1.0 ± 0.0 | 0.5 ± 0.2 | 1.7 ± 0.3† | 0.0 ± 0.0 |
| Epithelium | 1.5 ± 0.5 | 0.7 ± 0.2 | 2.9 ± 0.2* | 0.0 ± 0.0 |
| Total | 2.3 ± 0.8 | 1.2 ± 0.4 | 4.6 ± 0.4* | 0.0 ± 0.0 |
| Skin | 0.0 ± 0.0 | 0.0 ± 0.0 | 1.3 ± 0.2* | 0.0 ± 0.0 |
| Immune reconstitution | n = 3 | n = 4 | n = 4 | ND |
| Thymus, cells × 106 | ||||
| Total thymocytes | 82 ± 5 | 62 ± 13 | 1 ± 2 | ND |
| CD4 SP cells | 11 ± 1 | 8 ± 2 | 0 ± 0 | ND |
| CD8 SP cells | 2 ± 1 | 2 ± 1 | 0 ± 0 | ND |
| Spleen, cells × 106 | ||||
| CD4+ T cells | 21 ± 2 | 18 ± 2 | 8 ± 4† | ND |
| CD8+ T cells | 9 ± 1 | 6 ± 1 | 3 ± 1† | ND |
| B220+ cells | 43 ± 3 | 55 ± 5.8 | 22 ± 8.6† | ND |
The extent of GVHD was assessed 10 weeks after BMT. Liver, small intestine, large intestine, and skin were analyzed using the histopathologic scoring system described in “Materials and methods.” Thymus and spleens were phenotyped by a flow cytometric analysis. II-/- mice have normal counts of DP cells in the thymus and of CD8+ and B220+ cells in spleen.43 Data are expressed as mean ± SE. SP indicates single positive; ND, not done; and DP, double positive.
P < .001.
P < .01.