Intrinsic dissociation rates and reactive compliance values for the GPIbα−VWF-A1 tether bond
. | k0off, s−1 . | σ, nm . | R2 . |
|---|---|---|---|
| VWF-A1, WT | |||
| SPE | 3.21 ± 0.15 | 0.018 ± 0.002 | 0.97 |
| MCR | 3.45 ± 0.37 | 0.016 ± 0.002 | |
| Type 2B, lle546Val | |||
| SPE | 0.56 ± 0.02 | 0.026 ± 0.001 | 0.99 |
| MCR | 0.55 ± 0.03 | 0.029 ± 0.004 | |
| PT-VWD, Gly233Val | |||
| SPE | 0.55 ± 0.02 | 0.029 ± 0.001 | 0.98 |
| MCR | 0.67 ± 0.11 | 0.031 ± 0.005 |
. | k0off, s−1 . | σ, nm . | R2 . |
|---|---|---|---|
| VWF-A1, WT | |||
| SPE | 3.21 ± 0.15 | 0.018 ± 0.002 | 0.97 |
| MCR | 3.45 ± 0.37 | 0.016 ± 0.002 | |
| Type 2B, lle546Val | |||
| SPE | 0.56 ± 0.02 | 0.026 ± 0.001 | 0.99 |
| MCR | 0.55 ± 0.03 | 0.029 ± 0.004 | |
| PT-VWD, Gly233Val | |||
| SPE | 0.55 ± 0.02 | 0.029 ± 0.001 | 0.98 |
| MCR | 0.67 ± 0.11 | 0.031 ± 0.005 |
Values for k0off and σ were determined from regressions of data obtained from 2 independent analyses, MC simulation (MCR) and statistical point estimate (SPE). P values based on a 2-tailed Student t test revealed no statistically significant differences in MCR- or SPE-derived values for k0off and σ as well as for intrinsic off-rates (MCR) for PT and type 2B VWD (P >.1).