Table 1. The effect of PS-341 and TRAIL... | American Society of Hematology

Table 1.

The effect of PS-341 and TRAIL treatment on long-term growth of C1498

Treatment^*			Cell number, × 10⁶†
Inhibitor (nM)	TRAIL	2 days	7 days	11 days
0	0	7.8 ± 0.9	(7988)	—
0	+	2.7 ± 0.5	—	—
PS-341 (1)	0	7.2 ± 0.9	—	—
PS-341 (1)	+	2.7 ± 0.8	—	—
PS-341 (5)	0	8.8 ± 1.0	—	—
PS-341 (5)	+	4.2 ± 1.2	—	—
PS-341 (20)	0	BD	5.9 ± 1.2	(1510)
PS-341 (20)	+	BD	BD	7.1 ± 0.4

Treatment^*			Cell number, × 10⁶†
Inhibitor (nM)	TRAIL	2 days	7 days	11 days
0	0	7.8 ± 0.9	(7988)	—
0	+	2.7 ± 0.5	—	—
PS-341 (1)	0	7.2 ± 0.9	—	—
PS-341 (1)	+	2.7 ± 0.8	—	—
PS-341 (5)	0	8.8 ± 1.0	—	—
PS-341 (5)	+	4.2 ± 1.2	—	—
PS-341 (20)	0	BD	5.9 ± 1.2	(1510)
PS-341 (20)	+	BD	BD	7.1 ± 0.4

— indicates not determined; BD, below detection level of the MTS assay.

C1498 cells (1 × 10⁵) were incubated overnight in the presence or absence of PS-341 and TRAIL (1 μg/mL). Cells were then washed thoroughly and resuspended in 1 mL fresh media. Various dilutions of cells were transferred to microtiter plates.

†

Viable cell numbers were estimated at various time points using an MTS assay based on a standard curve with fixed numbers of C1498. The number in parentheses indicates a theoretical number assuming a 4-fold increase in cell number per day.

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