Oligonucleotide primers for AK PCR amplification and sequencing
Exon . | Name . | Amplification primer, 5′ to 3′ . | DMSO, % . | Annealing temperature, °C . | Fragment size, bp . |
|---|---|---|---|---|---|
| 1 | AK 1FN | GGTGTCGCCGCGCACCCG | 5 | 66 | 394 |
| AK 1RO | CGAGGCACAGATCCCCAGCG | ||||
| 2 | AK 2F | GCACACACCAGTTGCCTTGG | 5 | 66 | 271 |
| AK 2R | TGGAGGCTCCCAGCCAGG | ||||
| 3 to 4 | AK 3F | GCCTGGGACTCCAGAGGTG | 5 | 63 | 543 |
| AK 4R | AGCTTTGCCTGTGTGCCGTG | ||||
| 5 | AK 5FN | GCAGCGTGACGAGAGGCTG | 0 | 66 | 293 |
| AK 5R | GAACTCTGACCTGCATCACAG | ||||
| 6 | AK 6F | GAAGGGATTGAAGGTCTGAGG | 5 | 63 | 398 |
| AK 6R | AGAGGGGCTGGCCTGAGG | ||||
| 7 | AK 7F | AGGCAGGCCTGGTCTCTGG | 5 | 67 | 263 |
| AK 7R | GTCTGTGCTCAGCAGGGCAG |
Exon . | Name . | Amplification primer, 5′ to 3′ . | DMSO, % . | Annealing temperature, °C . | Fragment size, bp . |
|---|---|---|---|---|---|
| 1 | AK 1FN | GGTGTCGCCGCGCACCCG | 5 | 66 | 394 |
| AK 1RO | CGAGGCACAGATCCCCAGCG | ||||
| 2 | AK 2F | GCACACACCAGTTGCCTTGG | 5 | 66 | 271 |
| AK 2R | TGGAGGCTCCCAGCCAGG | ||||
| 3 to 4 | AK 3F | GCCTGGGACTCCAGAGGTG | 5 | 63 | 543 |
| AK 4R | AGCTTTGCCTGTGTGCCGTG | ||||
| 5 | AK 5FN | GCAGCGTGACGAGAGGCTG | 0 | 66 | 293 |
| AK 5R | GAACTCTGACCTGCATCACAG | ||||
| 6 | AK 6F | GAAGGGATTGAAGGTCTGAGG | 5 | 63 | 398 |
| AK 6R | AGAGGGGCTGGCCTGAGG | ||||
| 7 | AK 7F | AGGCAGGCCTGGTCTCTGG | 5 | 67 | 263 |
| AK 7R | GTCTGTGCTCAGCAGGGCAG |