Regulation of gene expression in CD3+ and CD4+ T cells upon G-CSF treatment in vivo
. | Name of gene . | GenBank accession no. . | CD3+ T cells, fold change . | CD4+ T cells, fold change . |
|---|---|---|---|---|
| Activation marker | ||||
| A | CD69 | NM001781 | 2.1 | 5.8 |
| B | CD53 | NM000560 | 1.6 | 1.4 |
| Cell-surface molecules/adhesion marker | ||||
| C | CD44H | AX060538 | 0.5 | 0.7 |
| D | CD5 | X04391 | 0.7 | 0.7 |
| E | LFA1-α | AF114104 | 0.4 | 0.3 |
| Transcription factors | ||||
| F | GATA-3 | NM002051 | 1.5 | 7.6 |
| G | Stat5 | NM003152 | 1.8 | 16 |
| H | ISGF3-γ | NM006984 | 0.6 | 0.7 |
. | Name of gene . | GenBank accession no. . | CD3+ T cells, fold change . | CD4+ T cells, fold change . |
|---|---|---|---|---|
| Activation marker | ||||
| A | CD69 | NM001781 | 2.1 | 5.8 |
| B | CD53 | NM000560 | 1.6 | 1.4 |
| Cell-surface molecules/adhesion marker | ||||
| C | CD44H | AX060538 | 0.5 | 0.7 |
| D | CD5 | X04391 | 0.7 | 0.7 |
| E | LFA1-α | AF114104 | 0.4 | 0.3 |
| Transcription factors | ||||
| F | GATA-3 | NM002051 | 1.5 | 7.6 |
| G | Stat5 | NM003152 | 1.8 | 16 |
| H | ISGF3-γ | NM006984 | 0.6 | 0.7 |
The regulation of gene expression in T cells upon G-CSF treatment in vivo was investigated in CD3+ and CD4+ T cells from healthy stem cell donors before and after G-CSF application. The results of cDNA array hybridizations are shown as mean factor of expression ratios (after vs before G-CSF) of regulated genes from 3 paired array experiments each for CD3+ and CD4+ T cells. Array analyses of CD3+ T cells were performed from individual donors before and after G-CSF application; CD4+ T cells had to be pooled for each array experiment from 3 stem cell donors in order to obtain sufficient starting material