NB1 mRNA copies in NB1 GP+ and GP- neutrophils
Healthy donors (% NB1 GP+) . | n . | Median NB1 mRNA formation NB1 GP+ (range) . | Median NB1 mRNA formation NB1 GP- (range) . | Median ratio (range) . |
|---|---|---|---|---|
| Unstimulated (20-81) | 5 | 3630 (2382-15 450) | 99 (77-308) | 28 (12-201) |
| After G-CSF stimulation (36) | 1 | 5 865 068 (3500 before G-CSF) | 223 069 (88 before G-CSF) | 26 |
| After G-CSF stimulation (slightly greater than 0) | 1 | 2475 (21 before G-CSF) | 2558 (21 before G-CSF) | 1 |
Healthy donors (% NB1 GP+) . | n . | Median NB1 mRNA formation NB1 GP+ (range) . | Median NB1 mRNA formation NB1 GP- (range) . | Median ratio (range) . |
|---|---|---|---|---|
| Unstimulated (20-81) | 5 | 3630 (2382-15 450) | 99 (77-308) | 28 (12-201) |
| After G-CSF stimulation (36) | 1 | 5 865 068 (3500 before G-CSF) | 223 069 (88 before G-CSF) | 26 |
| After G-CSF stimulation (slightly greater than 0) | 1 | 2475 (21 before G-CSF) | 2558 (21 before G-CSF) | 1 |
In healthy donors there is a clear difference between NB1 GP+ and NB1 GP- neutrophils. G-CSF administration causes a 1000-fold up-regulation of NB1 mRNA, but the ratio remains in the magnitude of untreated individuals. G-CSF does not up-regulate the NB1 mRNA content of neutrophils with an extremely low percentage of NB1 GP+ neutrophils. There is no difference between cells with the weakest and the strongest NB1 staining in immunofluorescence