TCL1 expression as detected by immunostaining and Western blot
Diagnosis . | TCL1+ (%) . |
|---|---|
| CD4+CD56+ blastic tumor | 10/12 (83)* |
| Nasal-type NK-cell lymphoma | 0/15 |
| Aggressive NK-cell leukemia | 0/1 |
| NK-cell large granular lymphocyte leukemia | 0/2 |
| Chronic myelogenous leukemia, 2 chronic phase, 2 T-cell blast crisis | 0/4 |
| Extramedullary myeloid cell tumors with other AML-M4 or M5 immunophenotypes† | 1/14 (7)† |
| Extramedullary myeloid cell tumors with other myeloid immunophenotypes | 0/7 |
| T-cell lymphoblastic lymphoma/leukemia | 1/7 (14)‡ |
| T-prolymphocytic leukemia | 30/40 (75)§ |
| Adult T-cell leukemia/lymphoma | 1/5 (20)† |
| T-cell large granular lymphocyte leukemia | 0/1 |
| Hepatosplenic T-cell lymphoma | 0/2 |
| Enteropathy-type T-cell lymphoma | 0/2 |
| Angioimmunoblastic lymphoma | 0/10 |
| ALCL, including 5 primary cutaneous ALCL | 0/21 |
| Nodal peripheral T-cell lymphoma, unspecified | 0/20 |
| Mycosis fungoides/Sézary syndrome | 0/36§ |
| Subcutaneous panniculitis-like T-cell lymphoma | 0/1 |
| Lymphomatoid papulosis | 0/6 |
| Cutaneous T-cell lymphoma, other types | 0/6 |
| Lymphomatoid granulomatosis | 0/2 |
| Hodgkin lymphoma | 0/10 |
Diagnosis . | TCL1+ (%) . |
|---|---|
| CD4+CD56+ blastic tumor | 10/12 (83)* |
| Nasal-type NK-cell lymphoma | 0/15 |
| Aggressive NK-cell leukemia | 0/1 |
| NK-cell large granular lymphocyte leukemia | 0/2 |
| Chronic myelogenous leukemia, 2 chronic phase, 2 T-cell blast crisis | 0/4 |
| Extramedullary myeloid cell tumors with other AML-M4 or M5 immunophenotypes† | 1/14 (7)† |
| Extramedullary myeloid cell tumors with other myeloid immunophenotypes | 0/7 |
| T-cell lymphoblastic lymphoma/leukemia | 1/7 (14)‡ |
| T-prolymphocytic leukemia | 30/40 (75)§ |
| Adult T-cell leukemia/lymphoma | 1/5 (20)† |
| T-cell large granular lymphocyte leukemia | 0/1 |
| Hepatosplenic T-cell lymphoma | 0/2 |
| Enteropathy-type T-cell lymphoma | 0/2 |
| Angioimmunoblastic lymphoma | 0/10 |
| ALCL, including 5 primary cutaneous ALCL | 0/21 |
| Nodal peripheral T-cell lymphoma, unspecified | 0/20 |
| Mycosis fungoides/Sézary syndrome | 0/36§ |
| Subcutaneous panniculitis-like T-cell lymphoma | 0/1 |
| Lymphomatoid papulosis | 0/6 |
| Cutaneous T-cell lymphoma, other types | 0/6 |
| Lymphomatoid granulomatosis | 0/2 |
| Hodgkin lymphoma | 0/10 |
ALCL indicates anaplastic large cell lymphoma.
Karyotypic analysis of 6 cases revealed 3 diploid karyotypes, 1 case with monosomy 5, another with monosomy 7, and a third with trisomy 8. No karyotypic abnormalities of chromosome 14 were noted. Multiple chromosomal losses were previously found in CD4+CD56+ tumors.7,22
TCL1 staining intensity was weak and variable with only focally nuclear staining. The weakly TCL1+ AML-M5 was CD56+ and negative for CD4 and CD123. Among extramedullary myeloid cell tumors with monocytic differentiation, 3 of 11 cases were CD56+ and all were negative for TdT.
The TCL1+ case of T-cell lymphoblastic lymphoma/leukemia was TdT+ and CD56-.
Immunostaining results were confirmed by TCL1 Western blot analysis in 14 T-PLL and 3 Sézary syndrome cases.