Differentiation of cultured cells into megakaryocytes and platelets
. | 3 day . | 8 day . | 13 day . | 18 day . |
|---|---|---|---|---|
| GPllb, CD41+ cells, % | 3.7 | 74.1 | 82.6 | 88.9 |
| GPlbα, CD42b+ cells, % | 2.0 | 60.2 | 82.6 | 90.1 |
. | 3 day . | 8 day . | 13 day . | 18 day . |
|---|---|---|---|---|
| GPllb, CD41+ cells, % | 3.7 | 74.1 | 82.6 | 88.9 |
| GPlbα, CD42b+ cells, % | 2.0 | 60.2 | 82.6 | 90.1 |
CD34+ progenitor cells from cord blood were isolated and cultured under conditions promoting megakaryocytopoiesis. About 90% of the cultured cells differentiated into megakaryocytes that were positive for CD41 or CD42b.