Cytokine/chemokine levels after Ptx treatment
Day . | WBC count, 1000/μL . | CFU-C level, no./mL . | IL-6 level, pg/mL . | IL-8 level, pg/mL . | MCP-1 level, pg/mL . | G-CSF level, pg/mL . | SCF level, pg/mL . | SDF-1α level, pg/mL . | SDF-1β level, pg/mL . |
|---|---|---|---|---|---|---|---|---|---|
| Monkey* | |||||||||
| PB | |||||||||
| -1 | 8.3 | 118.6 ± 9.0 | <2.0 | 119.0 | 130.0 | <2.0 | <120.0 | <160.0 | <120.0 |
| +1 | 43.0 | 254 ± 65.6 | <2.0 | 64.5 | <32.0 | <2.0 | 70.5 | <240.0 | <240.0 |
| +2 | 36.7 | 377.4 ± 52.9 | 436.6 | 121.6 | 362.8 | 170.9 | 47.6 | <240.0 | <240.0 |
| +3 | 64.0 | 5359 ± 705.6 | 2199.5 | 131.5 | 257.5 | 317.8 | 36.0 | <240.0 | <240.0 |
| BM | |||||||||
| -1 | ND | ND | <2.0 | 345.2 | 237.4 | 6.2 | <120.0 | 12 188.0 | 8319.5 |
| +3 | ND | ND | 1109.5 | 143.5 | 441.5 | 213.7 | <120.0 | 2948.3§ | 1948.8§ |
| Mouse† | |||||||||
| Baseline‡ | ND | ND | ND | ND | ND | ND | ND | 1856 ± 119 | <120.0 |
| PB, +3 | ND | ND | ND | ND | ND | ND | ND | 1349 ± 253 | <120.0 |
| BM, +3 | ND | ND | ND | ND | ND | ND | ND | 4539 ± 41∥ | 816 ± 64.9 |
Day . | WBC count, 1000/μL . | CFU-C level, no./mL . | IL-6 level, pg/mL . | IL-8 level, pg/mL . | MCP-1 level, pg/mL . | G-CSF level, pg/mL . | SCF level, pg/mL . | SDF-1α level, pg/mL . | SDF-1β level, pg/mL . |
|---|---|---|---|---|---|---|---|---|---|
| Monkey* | |||||||||
| PB | |||||||||
| -1 | 8.3 | 118.6 ± 9.0 | <2.0 | 119.0 | 130.0 | <2.0 | <120.0 | <160.0 | <120.0 |
| +1 | 43.0 | 254 ± 65.6 | <2.0 | 64.5 | <32.0 | <2.0 | 70.5 | <240.0 | <240.0 |
| +2 | 36.7 | 377.4 ± 52.9 | 436.6 | 121.6 | 362.8 | 170.9 | 47.6 | <240.0 | <240.0 |
| +3 | 64.0 | 5359 ± 705.6 | 2199.5 | 131.5 | 257.5 | 317.8 | 36.0 | <240.0 | <240.0 |
| BM | |||||||||
| -1 | ND | ND | <2.0 | 345.2 | 237.4 | 6.2 | <120.0 | 12 188.0 | 8319.5 |
| +3 | ND | ND | 1109.5 | 143.5 | 441.5 | 213.7 | <120.0 | 2948.3§ | 1948.8§ |
| Mouse† | |||||||||
| Baseline‡ | ND | ND | ND | ND | ND | ND | ND | 1856 ± 119 | <120.0 |
| PB, +3 | ND | ND | ND | ND | ND | ND | ND | 1349 ± 253 | <120.0 |
| BM, +3 | ND | ND | ND | ND | ND | ND | ND | 4539 ± 41∥ | 816 ± 64.9 |
ND indicates not determined.
In addition to the chemokines/cytokines listed, we also determined IL-1α, M-CSF, and GM-CSF levels, but these did not show any changes from pretreatment levels.
BM and PB values represent means from 4 animals.
Baseline levels were derived from PB of 15 BDF1 mice.
Significant reduction in SDF-1 level is seen; however, this sample was diluted with PB. For the same reason, it is unclear to what extent IL-6 and G-CSF are increased in BM.
When calculated per femur, this level is about 50% lower than baseline values obtained previously.32