Sequences of oligonucleotide primers and probes used for RT-PCR
Nested PCR | |
| First step | |
| BCR68 | 5′-AGAAGCTTCTCCCTGACATCCG-3′ |
| ABL3- | 5′-GGTACCAGGAGTGTTTCTCCAGACTG-3′ |
| BCR15 | 5′-CTCAGCTGTGTCCCTGTAGACG-3′ |
| Second step | |
| BCRfwd | 5′-TGAAACTCCAGACTGTCC-3′ |
| ABLrev | 5′-TCAGACCCTGAGGCTCAAAG-3′ |
| BCR20 | 5′-CTCCAGGGTGCAGTACAGA-3′ |
| Real-time quantitative PCR | |
| p210BCR/ABL | |
| Primer p210-F | 5′-CATTCCGCTGACCATCAATAA-3′ |
| Primer p210-R | 5′-AACGAGCGGCTTCACTCAGA-3′ |
| TaqMan probe | FAM-AGCGGCCAGTAGCATCTGACTTTGAGC-TAMRA |
| GAPDH | |
| Primer GAPDH-F | 5′-GAAGGTGAAGGTCGGAGTC-3′ |
| Primer GAPDH-R | 5′-GAAGATGGTGATGGGATTTC-3′ |
| TaqMan probe | FAM-CAAGCTTCCCGTTCTCAGCC-TAMRA |
Nested PCR | |
| First step | |
| BCR68 | 5′-AGAAGCTTCTCCCTGACATCCG-3′ |
| ABL3- | 5′-GGTACCAGGAGTGTTTCTCCAGACTG-3′ |
| BCR15 | 5′-CTCAGCTGTGTCCCTGTAGACG-3′ |
| Second step | |
| BCRfwd | 5′-TGAAACTCCAGACTGTCC-3′ |
| ABLrev | 5′-TCAGACCCTGAGGCTCAAAG-3′ |
| BCR20 | 5′-CTCCAGGGTGCAGTACAGA-3′ |
| Real-time quantitative PCR | |
| p210BCR/ABL | |
| Primer p210-F | 5′-CATTCCGCTGACCATCAATAA-3′ |
| Primer p210-R | 5′-AACGAGCGGCTTCACTCAGA-3′ |
| TaqMan probe | FAM-AGCGGCCAGTAGCATCTGACTTTGAGC-TAMRA |
| GAPDH | |
| Primer GAPDH-F | 5′-GAAGGTGAAGGTCGGAGTC-3′ |
| Primer GAPDH-R | 5′-GAAGATGGTGATGGGATTTC-3′ |
| TaqMan probe | FAM-CAAGCTTCCCGTTCTCAGCC-TAMRA |