Primers used for real-time RT-PCR
Gene . | Primer 1 (forward, 5′-3′) . | Primer 2 (reverse, 5′-3′) . |
|---|---|---|
| GAPDH | TCATCCATGACAACTTTGGTATCG | TGGCAGGTTTTTCTAGACGGC |
| L13740 | ATGGTGAAGGAAGTTGTCCGAAC | CCGGAGAGCAGGTCGTAGAAC |
| M34996 | AATTTGATGGAGATGAGGAGTTCTACG | AGCAGCGGTAGAGTTGTAGCGTT |
| U47635 | GGAACATGTACCATCAGTTTGATCG | TCGAAGAGCATCATTTACGGGTAG |
| M23178 | CCTGCTGCTTCAGCTACACCTC | TGGCTGCTCGTCTCAAAGTAGTC |
| M97935 | GTTACTTTCCCTGACATCATTCGC | GAATTCTACAGAGCCCACTATCCGA |
| Z23090 | CAGGACGAGCATGGCTACATCT | GGGATGGTGATCTCGTTGGAC |
| S76617 | CTTGGCTCGAATCATCGACAG | CATCAGGAGGACTCCAAACGAC |
| M13929 | CCAACAGGAACTATGACCTCGACTAC | CTCGAATTTCTTCCAGATATCCT |
| HT4899 | CTCCCTATCTACACTAACATCCCACG | TCCTTGTCCTGTGAGTATAAATC |
| X68836 | AACTGGCAGAACTACGCCGTAA | TGTATCCTCATCAAGGTATTTCG |
| X75042 | CGAACCCAATTTATGACAACCG | TTTTGTTTCTTTGCTTTATTGCCG |
| U16660 | GTAGAGTGCTTCAACAAGATTTCGAGA | CGTTGAAGGTCTCCTGGTATCG |
| U81556 | CCACTTGCCCCAGAGAAGCTA | AACCCGACAGCAAATAATGACTAGG |
| D90070 | TGGAAGTCGAGTGTGCTACTCAACT | AGATTCAGAAGTTTCTGCCGGAA |
| U37547 | TTCCAAGGTGTGAGTTCTTGATAC | AGAGCCATTCTGTTCTTCCGAAT |
| X75546 | CCCTATGACCCTTACCCGTATGA | CTGTGGCATTGTCAAAGACGC |
Gene . | Primer 1 (forward, 5′-3′) . | Primer 2 (reverse, 5′-3′) . |
|---|---|---|
| GAPDH | TCATCCATGACAACTTTGGTATCG | TGGCAGGTTTTTCTAGACGGC |
| L13740 | ATGGTGAAGGAAGTTGTCCGAAC | CCGGAGAGCAGGTCGTAGAAC |
| M34996 | AATTTGATGGAGATGAGGAGTTCTACG | AGCAGCGGTAGAGTTGTAGCGTT |
| U47635 | GGAACATGTACCATCAGTTTGATCG | TCGAAGAGCATCATTTACGGGTAG |
| M23178 | CCTGCTGCTTCAGCTACACCTC | TGGCTGCTCGTCTCAAAGTAGTC |
| M97935 | GTTACTTTCCCTGACATCATTCGC | GAATTCTACAGAGCCCACTATCCGA |
| Z23090 | CAGGACGAGCATGGCTACATCT | GGGATGGTGATCTCGTTGGAC |
| S76617 | CTTGGCTCGAATCATCGACAG | CATCAGGAGGACTCCAAACGAC |
| M13929 | CCAACAGGAACTATGACCTCGACTAC | CTCGAATTTCTTCCAGATATCCT |
| HT4899 | CTCCCTATCTACACTAACATCCCACG | TCCTTGTCCTGTGAGTATAAATC |
| X68836 | AACTGGCAGAACTACGCCGTAA | TGTATCCTCATCAAGGTATTTCG |
| X75042 | CGAACCCAATTTATGACAACCG | TTTTGTTTCTTTGCTTTATTGCCG |
| U16660 | GTAGAGTGCTTCAACAAGATTTCGAGA | CGTTGAAGGTCTCCTGGTATCG |
| U81556 | CCACTTGCCCCAGAGAAGCTA | AACCCGACAGCAAATAATGACTAGG |
| D90070 | TGGAAGTCGAGTGTGCTACTCAACT | AGATTCAGAAGTTTCTGCCGGAA |
| U37547 | TTCCAAGGTGTGAGTTCTTGATAC | AGAGCCATTCTGTTCTTCCGAAT |
| X75546 | CCCTATGACCCTTACCCGTATGA | CTGTGGCATTGTCAAAGACGC |
The expression levels of the genes selected from the microarray data analyses were validated and confirmed by real-time quantitative RT-PCR (LightCycler). Primers were designed with online software (WebPrimer, http://alces.med.umn.edu) and used in RT-PCR as described.