Fcγ receptor expression and phagocytosis in COS-1 cells transiently cotransfected with CD8/γ chain chimeras and FcγRI
. | Expression (MFI) . | . | . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | FcγRI . | . | . | CD8 . | . | . | Phagocytosis (PI) . | . | . | ||||||
| Experiment no. . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | ||||||
| FcγRI | |||||||||||||||
| +CD8-γ-γ | 107 | 130 | 111 | 201 | 139 | 139 | 14 | 32 | 16 | ||||||
| +CD8 CD8γ | 100 | 104 | 68 | 420 | 270 | 220 | 0 | 0 | 0 | ||||||
| +CD8-γ (Cys7/Asp11)-γ | 132 | 237 | 79 | 123 | 98 | 184 | 14 | 12 | 4 | ||||||
. | Expression (MFI) . | . | . | . | . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | FcγRI . | . | . | CD8 . | . | . | Phagocytosis (PI) . | . | . | ||||||
| Experiment no. . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | 1 . | 2 . | 3 . | ||||||
| FcγRI | |||||||||||||||
| +CD8-γ-γ | 107 | 130 | 111 | 201 | 139 | 139 | 14 | 32 | 16 | ||||||
| +CD8 CD8γ | 100 | 104 | 68 | 420 | 270 | 220 | 0 | 0 | 0 | ||||||
| +CD8-γ (Cys7/Asp11)-γ | 132 | 237 | 79 | 123 | 98 | 184 | 14 | 12 | 4 | ||||||
The structure of the CD8/γ chain chimeras is indicated in Figure 2. Three representative experiments are shown. Cell surface expression of transfected receptors, determined by flow cytometry using mAb 32.2 for FcγRI and anti-CD8 antibody for the CD8/γ chimeras, is presented as mean fluorescence intensity (MFI). For each experiment, 900-1000 cells were microscopically examined in a random fashion to determine the phagocytic index (PI).