The effect of TM sequences on cell surface expression of Fcγ receptor chimeras
Chimeras (EC-TM-CY) . | % Expression (MFI) . |
|---|---|
| RIIIA-RI-γ | 100% |
| RIIIA-RIIIA-γ* | 7% ± 6% |
| RIIIA-γ-γ† | 91% ± 8% |
| RI-RI-γ | 100% |
| RI-RIIIA-γ‡ | 14% ± 9% |
| RI-γ-γ§ | 72% ± 25% |
| FcγRI | 100% |
| RI-RIIIA-γ∥ | 11% ± 7% |
| RI-CD8-γ¶ | 95% |
| CD8-RI-RI | 100% |
| CD8-RIIIA-RIIIA | 18% |
Chimeras (EC-TM-CY) . | % Expression (MFI) . |
|---|---|
| RIIIA-RI-γ | 100% |
| RIIIA-RIIIA-γ* | 7% ± 6% |
| RIIIA-γ-γ† | 91% ± 8% |
| RI-RI-γ | 100% |
| RI-RIIIA-γ‡ | 14% ± 9% |
| RI-γ-γ§ | 72% ± 25% |
| FcγRI | 100% |
| RI-RIIIA-γ∥ | 11% ± 7% |
| RI-CD8-γ¶ | 95% |
| CD8-RI-RI | 100% |
| CD8-RIIIA-RIIIA | 18% |
mAb 32.2 was used for receptors bearing the FcγRI extracellular domain (EC), mAb 3G8 for receptors bearing the FcγRIIIA EC, and anti-CD8 antibody for receptors bearing the CD8 EC. Receptors are grouped according to EC, and MFIs are presented as the percent of the Fcγ receptor bearing the FcγRI TM (100%) within the group.
9 individual experiments were performed.
4 individual experiments were performed.
6 individual experiments were performed.
5 individual experiments were performed.
8 individual experiments were performed.
3 individual experiments were performed.