EBV tetramers used in this study
EBV antigen (coordinates) . | Presenting HLA allele . | Amino acid sequence . | Normal range, cells per mm3† . | Reference no. . |
|---|---|---|---|---|
| Latent EBV epitopes | ||||
| EBNA1 (407-417) | B*3501 | HPVGEADYFEY | 2 (<0.5-21) [18] | 30 |
| EBNA3A (379-387) | B*0702 | RPPIFIRRL | 2 (<0.5-5) [20] | 20 |
| EBNA3A (502-510) | B*0702 | VPAPAGPIV | 0.6 (<0.5-3) [20] | 20 |
| EBNA3A (325-333) | B*0801 | FLRGRAYGL | 1 (<0.5-28) [20] | 20,29 |
| EBNA3A (458-466) | B*3501 | YPLHEQHGM | 1 (<0.5-51) [16] | 30 |
| EBNA3B (399-408) | A*1101 | AVFDRKSDAK | 1 (<0.5-5) [9] | UR |
| EBNA3B (416-424) | A*1101 | IVTDSVIK | 0.6 (<0.5-3) [11] | UR |
| EBNA3C (284-293) | A*0201 | LLDFVRMGV | 1 (<0.5-8) [21] | 20 |
| LMP2 (426-434) | A*0201 | CLGLLTMV | 0.6 (<0.5-6) [22] | 20 |
| Lytic EBV epitopes | ||||
| BMLF1 (280-288) | A*0201 | GLCTLVAML | 1 (<0.5-18) [24] | 29 |
| BZLF1 (190-197) | B*0801 | RAKFKQLL | 3 (<0.5-79) [18] | 29 |
| BZLF1 (54-64) | B*3501 | EPLPQGQLTAY | 5 (<0.5-133) [17] | 31 |
EBV antigen (coordinates) . | Presenting HLA allele . | Amino acid sequence . | Normal range, cells per mm3† . | Reference no. . |
|---|---|---|---|---|
| Latent EBV epitopes | ||||
| EBNA1 (407-417) | B*3501 | HPVGEADYFEY | 2 (<0.5-21) [18] | 30 |
| EBNA3A (379-387) | B*0702 | RPPIFIRRL | 2 (<0.5-5) [20] | 20 |
| EBNA3A (502-510) | B*0702 | VPAPAGPIV | 0.6 (<0.5-3) [20] | 20 |
| EBNA3A (325-333) | B*0801 | FLRGRAYGL | 1 (<0.5-28) [20] | 20,29 |
| EBNA3A (458-466) | B*3501 | YPLHEQHGM | 1 (<0.5-51) [16] | 30 |
| EBNA3B (399-408) | A*1101 | AVFDRKSDAK | 1 (<0.5-5) [9] | UR |
| EBNA3B (416-424) | A*1101 | IVTDSVIK | 0.6 (<0.5-3) [11] | UR |
| EBNA3C (284-293) | A*0201 | LLDFVRMGV | 1 (<0.5-8) [21] | 20 |
| LMP2 (426-434) | A*0201 | CLGLLTMV | 0.6 (<0.5-6) [22] | 20 |
| Lytic EBV epitopes | ||||
| BMLF1 (280-288) | A*0201 | GLCTLVAML | 1 (<0.5-18) [24] | 29 |
| BZLF1 (190-197) | B*0801 | RAKFKQLL | 3 (<0.5-79) [18] | 29 |
| BZLF1 (54-64) | B*3501 | EPLPQGQLTAY | 5 (<0.5-133) [17] | 31 |
EBNA indicates EBV nuclear antigen; LMP, latent membrane protein; BMLF, BamHIM leftward reading frame; BZLF, BamHIZ leftward reading frame; and UR, D.V.B. and F.M., unpublished results.
Median (minimum-maximum) results obtained in healthy EBV carriers. The number of carriers tested for each EBV tetramer is shown between brackets.