Clot formation kinetics, clot strength, and clot lysis in wild-type and transgenic mice
. | Age, mo . | α-Angle . | Maximal amplitude, mm . | Clot lysis, % . |
|---|---|---|---|---|
| Wild-type | ||||
| 1 | 72.8 ± 6.7 | 82.5 ± 0.7 | 0 ± 0 | |
| 2 | 66.1 ± 13.1 | 79.4 ± 4.9 | 0.1 ± 0.3 | |
| 4 | 76.3 ± 0.8 | 79.8 ± 0.9 | 0 ± 0 | |
| 6 | 62.3 ± 6.5 | 76.8 ± 2.5 | 0 ± 0 | |
| 8 | 68.1 ± 7.1 | 77.6 ± 3.4 | 0 ± 0 | |
| Transgenic | ||||
| 1 | 70.0 ± 4.8 | 73.2 ± 4.9 | 2.7 ± 3.4 | |
| 2 | 54.5 ± 15.2 | 55.5 ± 4.8* | 12.2 ± 11.3 | |
| 4 | 40.3 ± 13.5† | 48.9 ± 13.7† | 14.7 ± 10.3 | |
| 6 | 30.1 ± 14.5* | 49.1 ± 7.4† | 4.5 ± 10.1 | |
| 8 | 32.5 ± 6.9† | 47.0 ± 15.7† | 9.7 ± 14.6 |
. | Age, mo . | α-Angle . | Maximal amplitude, mm . | Clot lysis, % . |
|---|---|---|---|---|
| Wild-type | ||||
| 1 | 72.8 ± 6.7 | 82.5 ± 0.7 | 0 ± 0 | |
| 2 | 66.1 ± 13.1 | 79.4 ± 4.9 | 0.1 ± 0.3 | |
| 4 | 76.3 ± 0.8 | 79.8 ± 0.9 | 0 ± 0 | |
| 6 | 62.3 ± 6.5 | 76.8 ± 2.5 | 0 ± 0 | |
| 8 | 68.1 ± 7.1 | 77.6 ± 3.4 | 0 ± 0 | |
| Transgenic | ||||
| 1 | 70.0 ± 4.8 | 73.2 ± 4.9 | 2.7 ± 3.4 | |
| 2 | 54.5 ± 15.2 | 55.5 ± 4.8* | 12.2 ± 11.3 | |
| 4 | 40.3 ± 13.5† | 48.9 ± 13.7† | 14.7 ± 10.3 | |
| 6 | 30.1 ± 14.5* | 49.1 ± 7.4† | 4.5 ± 10.1 | |
| 8 | 32.5 ± 6.9† | 47.0 ± 15.7† | 9.7 ± 14.6 |
At 1, 2, 4, 6, and 8 months, coagulation was assessed by computerized thromboelastography of native whole blood from transgenic and wild-type animals. The maximal amplitude, a parameter of clot strength, as well as the α-angle, indicating the kinetics of clot formation, were significantly decreased in transgenic mice aged 4 months and older. Clot lysis was virtually absent in wild-type blood, and only a small percentage of clot lysis was found in transgenic mice. Clot lysis (%): 100 — (amplitude [mm] at 30 minutes after maximal amplitude normalized to maximal amplitude [mm] × 100). Each group had 4 to 6 mice.
P < .01, †P < .001 compared with age-matched wild-type control group.