Cytokine profiles from MLRs stimulated by myDCs and pcDCs
. | HIV-1 plasma virus load . | . | . | Cytokine levels in myDC MLRs, pg/mL . | . | . | . | . | . | Cytokine levels in pcDC MLRs, pg/mL . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Controls/patients . | . | myDCs, no. . | SI . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . | pcDCs, no. . | SI . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . | ||||||
| c01 | NT | 32 000 | 45.0 | 20.7 | 3 441.2 | 6.8 | 12.6 | 16 000 | 10.9 | 6.5 | 216.4 | 1.8 | BDL | ||||||
| c02 | NT | 32 000 | 80.7 | 8.6 | 847.5 | 4.7 | 14.4 | 4 000 | 41.5 | 11.3 | 263.2 | 5.4 | 4.7 | ||||||
| c03 | NT | 16 000 | 126.3 | 4.2 | 337.0 | 3.9 | 2.4 | 8 000 | 94.6 | 3.8 | 481.6 | 4.9 | 5.1 | ||||||
| c04 | NT | 16 000 | 135.5 | 4.1 | 299.3 | 2.3 | 9.4 | 16 000 | 139.7 | 5.3 | 1 590.1 | 3.0 | 9.4 | ||||||
| c05 | NT | 16 000 | 68.6 | 3.4 | 157.8 | 4.5 | 7.2 | 16 000 | 68.6 | 5.2 | 1 514.9 | 3.5 | 7.2 | ||||||
| c06 | NT | 8 000 | 106.6 | 3.4 | 147.1 | 2.2 | 4.5 | 8 000 | 54.8 | 6.3 | 1 396.1 | 2.7 | 4.5 | ||||||
| Median, controls | NA | NA | 93.7 | 4.2 | 318.2 | 4.2 | 3.1 | NA | 61.7 | 5.8 | 938.8 | 3.3 | 4.9 | ||||||
| pt16 | 368 | 2 000 | 28.0 | 0.0 | 53.8 | 0.0 | BDL | 2 000 | 11.2 | 1.5 | 119.9 | 2.5 | 2.7 | ||||||
| pt33 | 2 841 | 4 000 | 14.6 | 4.1 | 99.4 | 2.9 | BDL | 4 000 | 31.8 | 5.6 | 1 151.7 | 2.3 | 4.5 | ||||||
| pt03 | 12 525 | 2 000 | 11.6 | 7.6 | 2.4 | 1.6 | BDL | 4 000 | 23.3 | 9.0 | 51.6 | 4.6 | 3.4 | ||||||
| pt18 | 12 960 | 2 000 | 18.1 | 2.8 | 84.2 | 3.2 | 2.7 | 2 000 | 14.7 | 0.0 | 151.3 | 0.0 | BDL | ||||||
| pt07 | 280 500 | 8 000 | 42.3 | 7.6 | 211.0 | 5.6 | BDL | 4 000 | 8.1 | 3.6 | 47.4 | 3.0 | BDL | ||||||
| pt99 | 41 755 | 2 000 | 4.2 | 5.9 | 12.4 | 2.8 | BDL | 2 000 | 2.9 | 0.0 | 14.2 | 3.5 | BDL | ||||||
| pt96 | 68 433 | 2 000 | 28.2 | NT | NT | NT | NT | 4 000 | 2.8 | NT | NT | NT | NT | ||||||
| pt12 | 81 954 | 1 500 | 68.0 | 1.9 | 121.8 | 2.2 | 3.3 | 3 000 | 35.4 | 4.2 | 691.9 | 2.7 | 2.2 | ||||||
| pt06 | 84 531 | 8 000 | 37.8 | 3.7 | 59.5 | 3.4 | 3.5 | 16 000 | 27.4 | 5.5 | 135.0 | 4.3 | 3.2 | ||||||
| pt13 | 85 434 | 1 500 | 1.5 | 0.0 | 6.9 | 3.2 | BDL | 1 500 | 15.2 | 0.0 | 20.8 | 2.8 | BDL | ||||||
| pt11 | 105 092 | 4 000 | 32.5 | 3.6 | 106.0 | 3.6 | 2.3 | 4 000 | 29.8 | 4.3 | 324.1 | 4.3 | 2.2 | ||||||
| pt88 | 190 920 | 6 000 | 67.1 | 2.9 | 230.3 | 3.7 | 4.0 | 2 000 | 1.0 | 0.0 | 2.4 | 2.7 | BDL | ||||||
| pt87 | 207 486 | 6 000 | 26.8 | 5.2 | 175.9 | 2.9 | 7.9 | 2 500 | 6.0 | 0.0 | 17.2 | 0.0 | BDL | ||||||
| pt10 | 390 158 | 4 000 | 27.3 | 4.8 | 45.4 | 2.3 | 2.7 | 2 000 | 12.0 | 0.0 | 13.6 | 0.0 | BDL | ||||||
| pt45 | 417 773 | 2 000 | 7.5 | 0.0 | 16.6 | 2.2 | 2.4 | 2 000 | 2.7 | 0.0 | 20.8 | 3.2 | 4.0 | ||||||
| pt92 | 441 000 | 1 500 | 75.2 | 7.4 | 742.2 | 3.5 | 7.5 | 4 000 | 26.2 | 5.3 | 623.1 | 3.5 | 4.5 | ||||||
| pt05 | 500 000 | 6 000 | 21.3 | 4.4 | 42.5 | 0.0 | BDL | 6 000 | 16.0 | 3.6 | 36.4 | 3.3 | BDL | ||||||
| Median, patients | 85 434 | NA | 27.3* | 3.9 | 71.9* | 2.9 | 2.3 | NA | 14.7* | 2.5* | 49.5* | 2.9 | 1.1* | ||||||
. | HIV-1 plasma virus load . | . | . | Cytokine levels in myDC MLRs, pg/mL . | . | . | . | . | . | Cytokine levels in pcDC MLRs, pg/mL . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Controls/patients . | . | myDCs, no. . | SI . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . | pcDCs, no. . | SI . | IL-2 . | IFN-γ . | IL-4 . | IL-10 . | ||||||
| c01 | NT | 32 000 | 45.0 | 20.7 | 3 441.2 | 6.8 | 12.6 | 16 000 | 10.9 | 6.5 | 216.4 | 1.8 | BDL | ||||||
| c02 | NT | 32 000 | 80.7 | 8.6 | 847.5 | 4.7 | 14.4 | 4 000 | 41.5 | 11.3 | 263.2 | 5.4 | 4.7 | ||||||
| c03 | NT | 16 000 | 126.3 | 4.2 | 337.0 | 3.9 | 2.4 | 8 000 | 94.6 | 3.8 | 481.6 | 4.9 | 5.1 | ||||||
| c04 | NT | 16 000 | 135.5 | 4.1 | 299.3 | 2.3 | 9.4 | 16 000 | 139.7 | 5.3 | 1 590.1 | 3.0 | 9.4 | ||||||
| c05 | NT | 16 000 | 68.6 | 3.4 | 157.8 | 4.5 | 7.2 | 16 000 | 68.6 | 5.2 | 1 514.9 | 3.5 | 7.2 | ||||||
| c06 | NT | 8 000 | 106.6 | 3.4 | 147.1 | 2.2 | 4.5 | 8 000 | 54.8 | 6.3 | 1 396.1 | 2.7 | 4.5 | ||||||
| Median, controls | NA | NA | 93.7 | 4.2 | 318.2 | 4.2 | 3.1 | NA | 61.7 | 5.8 | 938.8 | 3.3 | 4.9 | ||||||
| pt16 | 368 | 2 000 | 28.0 | 0.0 | 53.8 | 0.0 | BDL | 2 000 | 11.2 | 1.5 | 119.9 | 2.5 | 2.7 | ||||||
| pt33 | 2 841 | 4 000 | 14.6 | 4.1 | 99.4 | 2.9 | BDL | 4 000 | 31.8 | 5.6 | 1 151.7 | 2.3 | 4.5 | ||||||
| pt03 | 12 525 | 2 000 | 11.6 | 7.6 | 2.4 | 1.6 | BDL | 4 000 | 23.3 | 9.0 | 51.6 | 4.6 | 3.4 | ||||||
| pt18 | 12 960 | 2 000 | 18.1 | 2.8 | 84.2 | 3.2 | 2.7 | 2 000 | 14.7 | 0.0 | 151.3 | 0.0 | BDL | ||||||
| pt07 | 280 500 | 8 000 | 42.3 | 7.6 | 211.0 | 5.6 | BDL | 4 000 | 8.1 | 3.6 | 47.4 | 3.0 | BDL | ||||||
| pt99 | 41 755 | 2 000 | 4.2 | 5.9 | 12.4 | 2.8 | BDL | 2 000 | 2.9 | 0.0 | 14.2 | 3.5 | BDL | ||||||
| pt96 | 68 433 | 2 000 | 28.2 | NT | NT | NT | NT | 4 000 | 2.8 | NT | NT | NT | NT | ||||||
| pt12 | 81 954 | 1 500 | 68.0 | 1.9 | 121.8 | 2.2 | 3.3 | 3 000 | 35.4 | 4.2 | 691.9 | 2.7 | 2.2 | ||||||
| pt06 | 84 531 | 8 000 | 37.8 | 3.7 | 59.5 | 3.4 | 3.5 | 16 000 | 27.4 | 5.5 | 135.0 | 4.3 | 3.2 | ||||||
| pt13 | 85 434 | 1 500 | 1.5 | 0.0 | 6.9 | 3.2 | BDL | 1 500 | 15.2 | 0.0 | 20.8 | 2.8 | BDL | ||||||
| pt11 | 105 092 | 4 000 | 32.5 | 3.6 | 106.0 | 3.6 | 2.3 | 4 000 | 29.8 | 4.3 | 324.1 | 4.3 | 2.2 | ||||||
| pt88 | 190 920 | 6 000 | 67.1 | 2.9 | 230.3 | 3.7 | 4.0 | 2 000 | 1.0 | 0.0 | 2.4 | 2.7 | BDL | ||||||
| pt87 | 207 486 | 6 000 | 26.8 | 5.2 | 175.9 | 2.9 | 7.9 | 2 500 | 6.0 | 0.0 | 17.2 | 0.0 | BDL | ||||||
| pt10 | 390 158 | 4 000 | 27.3 | 4.8 | 45.4 | 2.3 | 2.7 | 2 000 | 12.0 | 0.0 | 13.6 | 0.0 | BDL | ||||||
| pt45 | 417 773 | 2 000 | 7.5 | 0.0 | 16.6 | 2.2 | 2.4 | 2 000 | 2.7 | 0.0 | 20.8 | 3.2 | 4.0 | ||||||
| pt92 | 441 000 | 1 500 | 75.2 | 7.4 | 742.2 | 3.5 | 7.5 | 4 000 | 26.2 | 5.3 | 623.1 | 3.5 | 4.5 | ||||||
| pt05 | 500 000 | 6 000 | 21.3 | 4.4 | 42.5 | 0.0 | BDL | 6 000 | 16.0 | 3.6 | 36.4 | 3.3 | BDL | ||||||
| Median, patients | 85 434 | NA | 27.3* | 3.9 | 71.9* | 2.9 | 2.3 | NA | 14.7* | 2.5* | 49.5* | 2.9 | 1.1* | ||||||
The cytokines detected by cytometric bead array in supernatants of mixed leukocyte reactions stimulated with myDCs and pcDCs for 5 days. Samples chosen for analysis were those that generated the highest proliferation. Cytokine levels are in picograms per milliliter. Median values are in bold. SI indicates stimulation index; c, control; BDL, below detection limit; NA, not available; NT, not tested.
P < .01 Mann Whitney U test.