Viral load in DCs and ability to stimulate T-lymphocyte proliferation
. | . | CD4 cell count . | Stimulation index . | . | Lowest no. of cells giving positive signal . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Patients . | Virus load . | . | pcDCs . | myDCs . | pcDCs . | myDCs . | T cells . | |||
| pt06 | 84 531 | 191 | 27.4 | 37.8 | 1 000 | 1 000 | 1 000 | |||
| pt11 | 105 092 | 174 | 29.8 | 32.5 | 200 | 1 000 | 40 | |||
| pt07 | 280 500 | 615 | 8.1 | 42.3 | 5 000 | 5 000 | 5 000 | |||
| pt10 | 390 158 | 213 | 12.0 | 27.3 | 200 | 200 | 40 | |||
| pt05 | 500 000 | 114 | 16.0 | 21.3 | 1 000 | 40 | 40 | |||
. | . | CD4 cell count . | Stimulation index . | . | Lowest no. of cells giving positive signal . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Patients . | Virus load . | . | pcDCs . | myDCs . | pcDCs . | myDCs . | T cells . | |||
| pt06 | 84 531 | 191 | 27.4 | 37.8 | 1 000 | 1 000 | 1 000 | |||
| pt11 | 105 092 | 174 | 29.8 | 32.5 | 200 | 1 000 | 40 | |||
| pt07 | 280 500 | 615 | 8.1 | 42.3 | 5 000 | 5 000 | 5 000 | |||
| pt10 | 390 158 | 213 | 12.0 | 27.3 | 200 | 200 | 40 | |||
| pt05 | 500 000 | 114 | 16.0 | 21.3 | 1 000 | 40 | 40 | |||
Five patients attended clinic and donated 50 mL blood on 2 separate occasions. One sample was used to assess the level of infection of DCs; the other was used in a mixed leukocyte reaction for assessment of function. In these patients, there was no correlation between the amount of virus detected within the DC populations and the ability to stimulate an MLR.