Characterization of anti-mIECSA and anti–DBL-γ3CSA mAbs by IFA and ELISA
| mAbs . | L-IFA . | AD-IFA . | ELISA . | |||||
|---|---|---|---|---|---|---|---|---|
| Isotype . | CSA . | CD36 . | ICAM-1 . | CSA . | CD36 . | ICAM-1 . | rDBL-γ3 . | |
| Anti-mIECSA | ||||||||
| 3F3/C2/C1 | IgG2a | + | − | − | + | − | − | + |
| 1H6/A4 | IgG1 | + | − | − | + | − | − | + |
| 2H5/F10 | IgM | + | − | − | + | − | − | − |
| 1E5/D6 | IgM | + | − | − | + | − | − | + |
| 1E5/E4 | IgM | + | − | − | + | + | + | + |
| 2A11/E4 | IgM | + | − | − | + | + | + | + |
| Anti-CHO-DBL-γ33 | ||||||||
| 1B4/D4 | IgG2a | + | − | − | + | − | − | + |
| 1B11/A5 | IgM | + | − | − | + | − | − | + |
| 1C5/D12 | IgM | + | − | − | + | − | − | − |
| 2F5/G10 | IgM | + | − | − | + | − | − | + |
| 4F10/C8 | IgM | + | − | − | + | − | − | − |
| mAbs . | L-IFA . | AD-IFA . | ELISA . | |||||
|---|---|---|---|---|---|---|---|---|
| Isotype . | CSA . | CD36 . | ICAM-1 . | CSA . | CD36 . | ICAM-1 . | rDBL-γ3 . | |
| Anti-mIECSA | ||||||||
| 3F3/C2/C1 | IgG2a | + | − | − | + | − | − | + |
| 1H6/A4 | IgG1 | + | − | − | + | − | − | + |
| 2H5/F10 | IgM | + | − | − | + | − | − | − |
| 1E5/D6 | IgM | + | − | − | + | − | − | + |
| 1E5/E4 | IgM | + | − | − | + | + | + | + |
| 2A11/E4 | IgM | + | − | − | + | + | + | + |
| Anti-CHO-DBL-γ33 | ||||||||
| 1B4/D4 | IgG2a | + | − | − | + | − | − | + |
| 1B11/A5 | IgM | + | − | − | + | − | − | + |
| 1C5/D12 | IgM | + | − | − | + | − | − | − |
| 2F5/G10 | IgM | + | − | − | + | − | − | + |
| 4F10/C8 | IgM | + | − | − | + | − | − | − |
Eleven mAbs have been analyzed in detail.
L-IFA indicates liquid immunofluorescence assay using intact IEs; AD-IFA, air-dried immunofluorescence assay of IEs; (+) positive; (−) negative.