Gene transfer efficiencies to various cell types in cultures of CD34+lin− and CD34+38−lin− human fetal liver cells after their supernatant transduction with a MSCV-HS2-β-globin-GFP virus
| Target cells . | % GFP+ total cells . | % GFP+ CD34+cells . | % GFP+ CD34+38−cells . | % GFP+ CFCs . | % GFP+ LTC-IC-derived CFCs . | % GFP+ glycophorin A+cells from 3-wk erythroid cultures . |
|---|---|---|---|---|---|---|
| CD34+lin− | 37 ± 5 | 19 ± 5 | ND | 53 ± 2 | ND | 36 ± 7 |
| (n = 3) | (n = 3) | (n = 3) | (n = 2) | |||
| CD34+38−lin− | 73 ± 6 | 47 ± 5 | 38 ± 9 | 58 ± 15 | 41 ± 27 | 36 ± 7 |
| (n = 5) | (n = 5) | (n = 5) | (n = 5) | (n = 3) | (n = 4) |
| Target cells . | % GFP+ total cells . | % GFP+ CD34+cells . | % GFP+ CD34+38−cells . | % GFP+ CFCs . | % GFP+ LTC-IC-derived CFCs . | % GFP+ glycophorin A+cells from 3-wk erythroid cultures . |
|---|---|---|---|---|---|---|
| CD34+lin− | 37 ± 5 | 19 ± 5 | ND | 53 ± 2 | ND | 36 ± 7 |
| (n = 3) | (n = 3) | (n = 3) | (n = 2) | |||
| CD34+38−lin− | 73 ± 6 | 47 ± 5 | 38 ± 9 | 58 ± 15 | 41 ± 27 | 36 ± 7 |
| (n = 5) | (n = 5) | (n = 5) | (n = 5) | (n = 3) | (n = 4) |
Values shown are the mean ± SEM percentages of the total number of cells or colonies expressing GFP after infection of lin-depleted cells (with or without removal of CD38+ cells as shown).
ND indicates not done.