Cytokine secretion by migratory MoDCs
| Migratory MoDC . | IL-1β† . | IL-6* . | IL-10† . | IL-12p70† . | n . |
|---|---|---|---|---|---|
| CD40L + IFN-γ + IL-1β | nd | 54.6 ± 31.5 | 0 ± 0 | 56 ± 53 | 2 |
| E coli | 26 ± 26 | 0.7 ± 0.1 | 9 ± 8 | 35 ± 35 | 3 |
| Migratory MoDC . | IL-1β† . | IL-6* . | IL-10† . | IL-12p70† . | n . |
|---|---|---|---|---|---|
| CD40L + IFN-γ + IL-1β | nd | 54.6 ± 31.5 | 0 ± 0 | 56 ± 53 | 2 |
| E coli | 26 ± 26 | 0.7 ± 0.1 | 9 ± 8 | 35 ± 35 | 3 |
IL-6 in ng/mL.
Concentration of cytokines in pg/mL.
MoDCs were prepared by culturing purified CD14+ monocytes for 7 days in GM-CSF and IL-4. On day 7, TNF-α, IFN-α, and PGE2 were added to induce the migratory differentiation pathway. After 2 days of stimulation the cultures were washed, and the indicated maturation and activation factors were then added for 3 days. Supernatants were examined for cytokines by ELISAs. Shown are the means ± SEM of n individual experiments.