Differentiation of hematopoietic cell lineages following ZAP-70 gene transfer
| . | No. . | WBCs per milliliter . | RBCs per microliter . | Splenic B cells (B220+/IgM+) . | Splenic myeloid cells (Mac-1+/Gr-1+) . |
|---|---|---|---|---|---|
| WT mice | 3 | ND | ND | 112.7 ± 10.6 | 3.2 ± 1.1 |
| Zap-70−/− mice | |||||
| EGFP transduced | 3 | 16.8 ± 2.2 | 8.5 ± 0.8 | 46.8 ± 40.7 | 32.6 ± 29.9 |
| Zap-70 transduced | 4 | 20.9 ± 2.8 | 7.6 ± 0.5 | 58.0 ± 24.6 | 3.0 ± 1.3 |
| WT-BM | 4 | 17.9 ± 5.1 | 8.3 ± 0.6 | 55.6 ± 15.8 | 4.9 ± 1.3 |
| . | No. . | WBCs per milliliter . | RBCs per microliter . | Splenic B cells (B220+/IgM+) . | Splenic myeloid cells (Mac-1+/Gr-1+) . |
|---|---|---|---|---|---|
| WT mice | 3 | ND | ND | 112.7 ± 10.6 | 3.2 ± 1.1 |
| Zap-70−/− mice | |||||
| EGFP transduced | 3 | 16.8 ± 2.2 | 8.5 ± 0.8 | 46.8 ± 40.7 | 32.6 ± 29.9 |
| Zap-70 transduced | 4 | 20.9 ± 2.8 | 7.6 ± 0.5 | 58.0 ± 24.6 | 3.0 ± 1.3 |
| WT-BM | 4 | 17.9 ± 5.1 | 8.3 ± 0.6 | 55.6 ± 15.8 | 4.9 ± 1.3 |
Peripheral blood samples were collected from the retro-orbital sinus 8 weeks post-BMT, and white blood cells (WBCs) and red blood cells (RBCs) were enumerated (× 106 ± SD). The numbers of B and myeloid cells in the spleens (× 106± SD) were determined at 18 weeks after transplantation following splenocyte staining with the indicated antibodies. Mean ± SD were obtained from 3 mice in each group.
ND indicates not done.