Splenocyte proliferation in ZAP-70–deficient mice following gene transfer
| . | Medium . | αCD3/αCD28 . | LPS . |
|---|---|---|---|
| WT mice | 702 ± 97 | 74 146 ± 1 821 | 61 841 ± 871 |
| ZAP-70−/− mice | |||
| EGFP transduced | 497 ± 84 | 1 424 ± 262 | 61 050 ± 2 660 |
| ZAP-70 transduced | 690 ± 163 | 11 553 ± 1 203 | 48 732 ± 3 944 |
| WT BM | 424 ± 51 | 58 456 ± 2 231 | 47 541 ± 2 392 |
| . | Medium . | αCD3/αCD28 . | LPS . |
|---|---|---|---|
| WT mice | 702 ± 97 | 74 146 ± 1 821 | 61 841 ± 871 |
| ZAP-70−/− mice | |||
| EGFP transduced | 497 ± 84 | 1 424 ± 262 | 61 050 ± 2 660 |
| ZAP-70 transduced | 690 ± 163 | 11 553 ± 1 203 | 48 732 ± 3 944 |
| WT BM | 424 ± 51 | 58 456 ± 2 231 | 47 541 ± 2 392 |
Splenocytes obtained from euthanized animals at 18 weeks after BMT were seeded in triplicate in 96-well flat-bottom plates. T- and B-cell proliferation were monitored in the presence of αCD3/αCD28 mAbs and LPS, respectively. Cells were cultured for 48 hours and pulsed with [3H]thymidine (0.5 μCi [18.5 KBq] per well) for the final 16 hours of culture. Cells were then harvested, incorporated radioactivity was determined on a scintillation counter (counts per minute), and mean values ± SD of triplicate cultures are shown. Data are representative of results obtained from 3 mice in each group.