Flow cytometric analysis showing CD47 reduction in 4.2(-) and Rh variant red cells
| . | . | RhD-neg, SABC . | RhD-pos, SABC . | Rhnull (reg), SABC . | Rhnull (amorph), SABC . | 4.2(-), SABC . | D--, SABC . | D.., SABC . | RN, SABC . |
|---|---|---|---|---|---|---|---|---|---|
| Protein | MAb | ||||||||
| Rh | BRIC69 | 130 | 145 | 0 | 0 | 140.0 | 100.0 | 81.5 | 142.0 |
| RhAG | LA18.18 | 120 | 114 | 0 | 21.6 | 119.0 | 93.0 | 61.0 | 118.0 |
| CD47 | 6H9 | 28 | 28 | 2.2 | 2.2 | 6.6 | 6.2 | 6.2 | 17.4 |
| CD47 | 3E12* | 20 | 19 | 2.1 | 2.1 | 5.5 | 5.4 | 5.2 | 12.3 |
| . | . | RhD-neg, SABC . | RhD-pos, SABC . | Rhnull (reg), SABC . | Rhnull (amorph), SABC . | 4.2(-), SABC . | D--, SABC . | D.., SABC . | RN, SABC . |
|---|---|---|---|---|---|---|---|---|---|
| Protein | MAb | ||||||||
| Rh | BRIC69 | 130 | 145 | 0 | 0 | 140.0 | 100.0 | 81.5 | 142.0 |
| RhAG | LA18.18 | 120 | 114 | 0 | 21.6 | 119.0 | 93.0 | 61.0 | 118.0 |
| CD47 | 6H9 | 28 | 28 | 2.2 | 2.2 | 6.6 | 6.2 | 6.2 | 17.4 |
| CD47 | 3E12* | 20 | 19 | 2.1 | 2.1 | 5.5 | 5.4 | 5.2 | 12.3 |
Specific antibody binding capacity (SABC) values (× 10−3).
Similar values were obtained with the B6H12, 5A3, and F8C10 anti-CD47 mAbs (not shown).