Flow cytometric analysis of 4.2(-)HS RBCs for expression of Rh complex proteins and various blood group-carrying proteins
| Protein . | . | RhD-negative (SD) . | RhD-positive (SD) . | 4.2(-)Lisboa (SD) . | 4.2(-)Nancy (SD) . |
|---|---|---|---|---|---|
| MAb* | SABC | SABC | SABC | SABC | |
| Rh | BRIC69 | 135 200 (± 283) | 131 500 (± 7 778) | 111 500 (± 707) | 115 000 (± 657) |
| RhAG | LA18.18 | 90 450 (± 2 192) | 87 500 (± 10 182) | 109 500 (± 3 536) | 111 000 (± 4 243) |
| CD47 | 6H9 | 26 150 (± 3 182) | 23 600 (± 1 980) | 5 800 (± 141) | 5 400 (± 10) |
| CD47 | 3E12 | 19 400 (± 1 556) | 18 350 (± 1 626) | 4 750 (± 71) | 4 200 (± 141) |
| LW | BS56 | 615 (± 120) | 1 525 (± 106) | 2 050 (± 71) | 100 (± 71) |
| Band 3 | BRIC6 | 408 000 (± 15 156) | 384 500 (± 23 335) | 351 000 (± 14 142) | 349 500 (± 4 950) |
| GPA | R18 | 357 500 (± 707) | 337 000 (± 19 799) | 302 000 (± 5 657) | 288 000 (± 7 071) |
| GPC | MR4-130 | 51 650 (± 1 909) | 49 100 (± 8 344) | 61 700 (± 990) | 55 000 (± 1 414) |
| DARC | anti-Fy6 2C3 | 2 500 (± 283) | 3 250 (± 778) | 5 050 (± 212) | 3 950 (± 212) |
| Kell | anti-k F7 | 2 250 (± 71) | 4 050 (± 71) | 4 900 (± 141) | 4 450 (± 212) |
| CD44 | 9D6 | 4 150 (± 71) | 3 950 (± 636) | 13 600 (± 283) | 11 200 (± 283) |
| Lu | F241 | 1 300 (± 141) | 1 700 (± 283) | 1 800 (± 141) | 1 250 (± 71) |
| PAb† | MFI | MFI | MFI | MFI | |
| AQP1 | anti-Co3 | 256 (± 15) | 243 (± 48) | 368 (± 18) | 240 (± 33) |
| GPB | anti-S | 2 (± 0) | 39 (± 23) | 41 (± 23) | 5 (± 1) |
| GPB | anti-s | 243 (± 10) | 66 (± 2) | 41 (± 2) | 231 (± 16) |
| JK | anti-Jk3 | 68 (± 12) | 94 (± 0) | 94 (± 25) | 92 (± 26) |
| Protein . | . | RhD-negative (SD) . | RhD-positive (SD) . | 4.2(-)Lisboa (SD) . | 4.2(-)Nancy (SD) . |
|---|---|---|---|---|---|
| MAb* | SABC | SABC | SABC | SABC | |
| Rh | BRIC69 | 135 200 (± 283) | 131 500 (± 7 778) | 111 500 (± 707) | 115 000 (± 657) |
| RhAG | LA18.18 | 90 450 (± 2 192) | 87 500 (± 10 182) | 109 500 (± 3 536) | 111 000 (± 4 243) |
| CD47 | 6H9 | 26 150 (± 3 182) | 23 600 (± 1 980) | 5 800 (± 141) | 5 400 (± 10) |
| CD47 | 3E12 | 19 400 (± 1 556) | 18 350 (± 1 626) | 4 750 (± 71) | 4 200 (± 141) |
| LW | BS56 | 615 (± 120) | 1 525 (± 106) | 2 050 (± 71) | 100 (± 71) |
| Band 3 | BRIC6 | 408 000 (± 15 156) | 384 500 (± 23 335) | 351 000 (± 14 142) | 349 500 (± 4 950) |
| GPA | R18 | 357 500 (± 707) | 337 000 (± 19 799) | 302 000 (± 5 657) | 288 000 (± 7 071) |
| GPC | MR4-130 | 51 650 (± 1 909) | 49 100 (± 8 344) | 61 700 (± 990) | 55 000 (± 1 414) |
| DARC | anti-Fy6 2C3 | 2 500 (± 283) | 3 250 (± 778) | 5 050 (± 212) | 3 950 (± 212) |
| Kell | anti-k F7 | 2 250 (± 71) | 4 050 (± 71) | 4 900 (± 141) | 4 450 (± 212) |
| CD44 | 9D6 | 4 150 (± 71) | 3 950 (± 636) | 13 600 (± 283) | 11 200 (± 283) |
| Lu | F241 | 1 300 (± 141) | 1 700 (± 283) | 1 800 (± 141) | 1 250 (± 71) |
| PAb† | MFI | MFI | MFI | MFI | |
| AQP1 | anti-Co3 | 256 (± 15) | 243 (± 48) | 368 (± 18) | 240 (± 33) |
| GPB | anti-S | 2 (± 0) | 39 (± 23) | 41 (± 23) | 5 (± 1) |
| GPB | anti-s | 243 (± 10) | 66 (± 2) | 41 (± 2) | 231 (± 16) |
| JK | anti-Jk3 | 68 (± 12) | 94 (± 0) | 94 (± 25) | 92 (± 26) |