Table 2.

Replating potential of HPP-CFCs derived from day 6 EBs

Wild typeSmad5−/−
Secondary replating   
 Individual replating   
  No. of primary HPP-CFCs replated 24 48  
  No. of secondary HPP-CFCs*  3.4 19.7  
 Bulk replating   
  No. of secondary HPP-CFCs 8 ± 3 37 ± 8  
Tertiary replating   
  No. of tertiary HPP-CFCs  0  51 ± 4 
Wild typeSmad5−/−
Secondary replating   
 Individual replating   
  No. of primary HPP-CFCs replated 24 48  
  No. of secondary HPP-CFCs*  3.4 19.7  
 Bulk replating   
  No. of secondary HPP-CFCs 8 ± 3 37 ± 8  
Tertiary replating   
  No. of tertiary HPP-CFCs  0  51 ± 4 

Results are from a representative experiment and reproduced in 3 independent experiments.

*

Average number of secondary HPP-CFCs per replated primary HPP-CFCs.

Results (mean ± SD) are from 3 independent cultures in a representative experiment.

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