Maximal rates of generation of fXa and thrombin in the intrinsic pathway by oxLDL-treated cells
| Cell type . | Rate of fXa generation, V (nM/min) . | Rate of thrombin generation, Vthr (nM/min) . | ||||
|---|---|---|---|---|---|---|
| Control, V0 . | oxLDL-treated, V . | V/V0 ratio . | Control, Vthr0 . | OxLDL-treated, Vthr . | Vthr/Vthr0ratio . | |
| Macrophages | 0.218 ± 0.05 | 1.768 ± 0.022 | 8.1 ± 1.8 | 16.6 ± 0.43 | 326 ± 11.2 | 19.6 ± 0.84 |
| SMCs | 0.176 ± 0.016 | 1.06 ± 0.056 | 6.0 ± 0.63 | 18.3 ± 0.6 | 187.5 ± 4.7 | 10.2 ± 0.42 |
| HAECs | 0.33 ± 0.012 | 0.51 ± 0.035 | 1.55 ± 0.12 | 33.5 ± 0.82 | 74 ± 3.1 | 2.2 ± 0.11 |
| Cell type . | Rate of fXa generation, V (nM/min) . | Rate of thrombin generation, Vthr (nM/min) . | ||||
|---|---|---|---|---|---|---|
| Control, V0 . | oxLDL-treated, V . | V/V0 ratio . | Control, Vthr0 . | OxLDL-treated, Vthr . | Vthr/Vthr0ratio . | |
| Macrophages | 0.218 ± 0.05 | 1.768 ± 0.022 | 8.1 ± 1.8 | 16.6 ± 0.43 | 326 ± 11.2 | 19.6 ± 0.84 |
| SMCs | 0.176 ± 0.016 | 1.06 ± 0.056 | 6.0 ± 0.63 | 18.3 ± 0.6 | 187.5 ± 4.7 | 10.2 ± 0.42 |
| HAECs | 0.33 ± 0.012 | 0.51 ± 0.035 | 1.55 ± 0.12 | 33.5 ± 0.82 | 74 ± 3.1 | 2.2 ± 0.11 |
After incubation of the cells with oxLDL for 12 hours, generation of fXa and thrombin was determined as described in “Materials and methods.” Maximal rates were determined from individual kinetic curves shown in Figures 1 and 7 by linear regression of fXa or thrombin concentration over time using a SigmaPlot 1.02 computer program.