Frequencies of progenitor cells in the tissues from progressively older WT and GATA-1low (KD) littermates
| . | Age, mo . | |||||
|---|---|---|---|---|---|---|
| 4-8 . | 8-12 . | 15-20 . | ||||
| WT . | KD . | WT . | KD . | WT . | KD . | |
| Spleen | ||||||
| CFU-E | 28 ± 6 | 233 ± 283-150 | 34 ± 9 | 313 ± 353-150 | 27 ± 7 | 152 ± 503-150 |
| BFU-E | 15 ± 3 | 110 ± 213-150 | 21 ± 6 | 97 ± 183-150 | 17 ± 5 | 37 ± 16 |
| CFU-GM | 40 ± 5 | 56 ± 16 | 36 ± 11 | 43 ± 4 | 41 ± 17 | 58 ± 13 |
| CFU-Mkp | 5 ± 1 | 6 ± 2 | 12 ± 3 | 10 ± 4 | 9 ± 3 | 62 ± 63-150 |
| CFU-Mkm | 2 ± 1 | 25 ± 53-151 | 3 ± 1 | 28 ± 63-151 | 4 ± 2 | 80 ± 153-150 |
| Single Mk cells | 7 ± 4 | 89 ± 143-151 | 11 ± 6 | 35 ± 33-151 | 9 ± 2 | 21 ± 6 |
| Bone marrow | ||||||
| CFU-E | 87 ± 20 | 381 ± 453-150 | 59 ± 19 | 326 ± 513-150 | 66 ± 23 | 100 ± 25 |
| BFU-E | 149 ± 33 | 165 ± 33 | 90 ± 12 | 105 ± 14 | 112 ± 21 | 26 ± 93-151 |
| CFU-GM | 165 ± 25 | 145 ± 17 | 187 ± 34 | 107 ± 13 | 149 ± 23 | 82 ± 113-151 |
| CFU-Mkp | 22 ± 4 | 4 ± 13-151 | 25 ± 7 | 3 ± 13-151 | 29 ± 11 | 5 ± 13-151 |
| CFU-Mkm | 10 ± 3 | 30 ± 73-151 | 7 ± 3 | 34 ± 53-151 | 10 ± 5 | 28 ± 8 |
| Single Mk cells | 17 ± 9 | 45 ± 153-151 | 12 ± 7 | 56 ± 233-151 | 15 ± 7 | 67 ± 173-151 |
| Peripheral blood | ||||||
| CFU-E | 0 | 6 ± 33-151 | 0 | 114 ± 133-150 | 0 | 26 ± 23-151 |
| BFU-E | 1 ± 1 | 4 ± 2 | 2 ± 2 | 12 ± 23-151 | 4 ± 3 | 34 ± 123-151 |
| CFU-GM | 3 ± 2 | 6 ± 4 | 4 ± 2 | 18 ± 63-151 | 5 ± 3 | 22 ± 53-151 |
| CFU-Mkp | 0 | 0 | 0 | 0 | 0 | 2 ± 1 |
| CFU-Mkm | 0 | 0 | 0 | 7 ± 3 | 0 | 15 ± 53-151 |
| Single Mk cells | 0 | 0 | 0 | 12 ± 2 | 0 | 19 ± 33-151 |
| . | Age, mo . | |||||
|---|---|---|---|---|---|---|
| 4-8 . | 8-12 . | 15-20 . | ||||
| WT . | KD . | WT . | KD . | WT . | KD . | |
| Spleen | ||||||
| CFU-E | 28 ± 6 | 233 ± 283-150 | 34 ± 9 | 313 ± 353-150 | 27 ± 7 | 152 ± 503-150 |
| BFU-E | 15 ± 3 | 110 ± 213-150 | 21 ± 6 | 97 ± 183-150 | 17 ± 5 | 37 ± 16 |
| CFU-GM | 40 ± 5 | 56 ± 16 | 36 ± 11 | 43 ± 4 | 41 ± 17 | 58 ± 13 |
| CFU-Mkp | 5 ± 1 | 6 ± 2 | 12 ± 3 | 10 ± 4 | 9 ± 3 | 62 ± 63-150 |
| CFU-Mkm | 2 ± 1 | 25 ± 53-151 | 3 ± 1 | 28 ± 63-151 | 4 ± 2 | 80 ± 153-150 |
| Single Mk cells | 7 ± 4 | 89 ± 143-151 | 11 ± 6 | 35 ± 33-151 | 9 ± 2 | 21 ± 6 |
| Bone marrow | ||||||
| CFU-E | 87 ± 20 | 381 ± 453-150 | 59 ± 19 | 326 ± 513-150 | 66 ± 23 | 100 ± 25 |
| BFU-E | 149 ± 33 | 165 ± 33 | 90 ± 12 | 105 ± 14 | 112 ± 21 | 26 ± 93-151 |
| CFU-GM | 165 ± 25 | 145 ± 17 | 187 ± 34 | 107 ± 13 | 149 ± 23 | 82 ± 113-151 |
| CFU-Mkp | 22 ± 4 | 4 ± 13-151 | 25 ± 7 | 3 ± 13-151 | 29 ± 11 | 5 ± 13-151 |
| CFU-Mkm | 10 ± 3 | 30 ± 73-151 | 7 ± 3 | 34 ± 53-151 | 10 ± 5 | 28 ± 8 |
| Single Mk cells | 17 ± 9 | 45 ± 153-151 | 12 ± 7 | 56 ± 233-151 | 15 ± 7 | 67 ± 173-151 |
| Peripheral blood | ||||||
| CFU-E | 0 | 6 ± 33-151 | 0 | 114 ± 133-150 | 0 | 26 ± 23-151 |
| BFU-E | 1 ± 1 | 4 ± 2 | 2 ± 2 | 12 ± 23-151 | 4 ± 3 | 34 ± 123-151 |
| CFU-GM | 3 ± 2 | 6 ± 4 | 4 ± 2 | 18 ± 63-151 | 5 ± 3 | 22 ± 53-151 |
| CFU-Mkp | 0 | 0 | 0 | 0 | 0 | 2 ± 1 |
| CFU-Mkm | 0 | 0 | 0 | 7 ± 3 | 0 | 15 ± 53-151 |
| Single Mk cells | 0 | 0 | 0 | 12 ± 2 | 0 | 19 ± 33-151 |
Results are presented as number of colonies per 105mononuclear cells (either from marrow or spleen) and per 10 μL peripheral blood. Mean (± SD) of at least 3 separate experiments performed in duplicate for a total of 3 to 5 mutants and 3 normal littermates were analyzed per each age group. Values in the GATA-1low mice statistically different from those observed in the corresponding wild-type animals are indicated by
(P > .01) and
(P < .05). Individual progenitor cells are indicated as CFU-E, BFU-E, CFU-GM, pure (p) or mixed (m) colony-forming unit, megakaryocytic, CFU-Mkp, and CFU-Mkm.