SCF-induced effects on co-stimulation of BFU-E and CFU-GM in Slug−/− mice
| Cytokines . | Cells . | |
|---|---|---|
| BFU-E/0.75 × 105 | ||
| Slug +/+ | Slug −/− | |
| EPO | 0 | 0 |
| EPO + SCF | 14.3 ± 0.9 | 3.1 ± 0.4 |
| CFU-GM/1 × 105 | ||
| Slug +/+ | Slug −/− | |
| GM-CSF | 40.3 ± 2.7 | 46.2 ± 2.9 |
| GM-CSF + SCF | 59.2 ± 3.2 | 48.9 ± 3.7 |
| Cytokines . | Cells . | |
|---|---|---|
| BFU-E/0.75 × 105 | ||
| Slug +/+ | Slug −/− | |
| EPO | 0 | 0 |
| EPO + SCF | 14.3 ± 0.9 | 3.1 ± 0.4 |
| CFU-GM/1 × 105 | ||
| Slug +/+ | Slug −/− | |
| GM-CSF | 40.3 ± 2.7 | 46.2 ± 2.9 |
| GM-CSF + SCF | 59.2 ± 3.2 | 48.9 ± 3.7 |
Murine BM cells from Slug+/+ and −/− mice were cultured in semisolid media in the presence of a combination of growth factors. Cytokines were used at the following concentrations: EPO, 2 U/mL; SCF, 100 ng/mL; GM-CSF, 10 ng/mL. Values are mean ± SEM of triplicate experiments. Differences between Slug+/+ and −/− were statistically significant (P < .0001), as determined by Mann-WhitneyU test.