Semiquantitative analysis of PMN spreading over neuroblastoma cells during ADCC
| Condition . | Tumor cells with attached and spread PMNs (%) . | |
|---|---|---|
| 30 min . | 120 min . | |
| Control | 0 | 0 |
| mAb hu14.18 | 0 | 0 |
| IC hu14.18/GM-CSF | 80 | 90 |
| IC + anti-CD18 | 0 | 0 |
| IC + anti-CD11b | 0 | 0 |
| Condition . | Tumor cells with attached and spread PMNs (%) . | |
|---|---|---|
| 30 min . | 120 min . | |
| Control | 0 | 0 |
| mAb hu14.18 | 0 | 0 |
| IC hu14.18/GM-CSF | 80 | 90 |
| IC + anti-CD18 | 0 | 0 |
| IC + anti-CD11b | 0 | 0 |
Conditions are the same as indicated in Figures 3 and 4.
Percentages are of tumor cells per 10 counted for which at least 30% of the cell surface was covered by a single PMN. Analysis was performed using electron microscopy as described in Figures 3 and 4.