Primers used to construct GPIbβ truncations
| N-terminal truncation vector* . | Left-half antisense primer . | Right-half sense primer . | ||
|---|---|---|---|---|
| Name . | Sequence . | Name . | Sequence . | |
| GPIbβΔ1-14 | aGPIbβ 97-80 | 5′-CGTCTCAACCTGCGGCCGGGCGGCT | SGPIbβ 140 | 5′-CGTCTCCAGGTGGGCGCCGCGGGCTGACTTGG |
| GPIbβΔ1-26 | aGPIbβ 99-82 | 5′-CGTCTCGGCACCTGCGGCCGGGCGGCT | SGPIbβ 176 | 5′-CGTCTCCGTGCCTTCCCTGTCGACACAACC |
| GPIbβΔ1-31 | aGPIbβ 97-80 | 5′-CGTCTCAACCTGCGGCCGGGCGGCT | SGPIbβ 191 | 5′-CGTCTCCAGGTACAACCGAGCTGGTGCTGACC |
| GPIbβ Δ1-58 | aGPIbβ 99-82 | 5′-CGTCTCTGGCACCTGCGGCCGGGCGGCT | SGPIbβ 272 | 5′-CGTCTCCGTGCACACCTGGGCGCCAcC |
| GPIbβ Δ1-65 | aGPIbβ 97-80 | 5′-CGTCTCAACCTGCGGCCGGGCGGCT | SGPIbβ 293 | 5′-CGTCTCCAGGTTGGCGCTGCGACTGCCGCCTTG |
| N-terminal truncation vector* . | Left-half antisense primer . | Right-half sense primer . | ||
|---|---|---|---|---|
| Name . | Sequence . | Name . | Sequence . | |
| GPIbβΔ1-14 | aGPIbβ 97-80 | 5′-CGTCTCAACCTGCGGCCGGGCGGCT | SGPIbβ 140 | 5′-CGTCTCCAGGTGGGCGCCGCGGGCTGACTTGG |
| GPIbβΔ1-26 | aGPIbβ 99-82 | 5′-CGTCTCGGCACCTGCGGCCGGGCGGCT | SGPIbβ 176 | 5′-CGTCTCCGTGCCTTCCCTGTCGACACAACC |
| GPIbβΔ1-31 | aGPIbβ 97-80 | 5′-CGTCTCAACCTGCGGCCGGGCGGCT | SGPIbβ 191 | 5′-CGTCTCCAGGTACAACCGAGCTGGTGCTGACC |
| GPIbβ Δ1-58 | aGPIbβ 99-82 | 5′-CGTCTCTGGCACCTGCGGCCGGGCGGCT | SGPIbβ 272 | 5′-CGTCTCCGTGCACACCTGGGCGCCAcC |
| GPIbβ Δ1-65 | aGPIbβ 97-80 | 5′-CGTCTCAACCTGCGGCCGGGCGGCT | SGPIbβ 293 | 5′-CGTCTCCAGGTTGGCGCTGCGACTGCCGCCTTG |
The BsmBI site is in underlined italics; the overhang is in bold.
GP indicates glycoprotein.
Numbering is as follows: Amino acid 1 is the first amino acid following the signal peptide. The “new” N-terminus follows immediately after the signal peptide.