There are no differences in expression of theBCR-ABL transcript between patients with and without deletions
| Patient/cell line . | Translocation type . | Transcript type . | BCR-ABLCT . | B2MCT . | ΔCT . | BCR-ABLtranscripts, % of control gene . |
|---|---|---|---|---|---|---|
| Deleted cell line | ||||||
| MC3 | Cl | b3a2 | 24.5 (± 0.1) | 20.5 (± 0.01) | 4 | 6.9 |
| Patients with deletions | ||||||
| DS63 | Cl | b3a2 | 34.5 (± 0.5) | 27.0 (± 0.3) | 7.5 | 0.7 |
| JD66 | Co | b2a2 | 28.3 (± 0.1) | 22.2 (± 0.04) | 6.1 | 1.9 |
| MF68 | Cl | b3a2 | 38.5 (± 0.7) | 30.6 (± 0.1) | 7.9 | 0.5 |
| SP68 | Cl | b2a2 | 29.2 (± 0.2) | 24.7 (± 0.2) | 4.5 | 5.2 |
| Mean for patients with deletions | 6.5 | 1.4 | ||||
| Median for patients with deletions | 6.8 (4.9-7.8)* | 1.1 (4.0-0.6)* | ||||
| Nondeleted cell lines | ||||||
| MEG-01 | Cl | b2a2 | 24.1 (± 0.06) | 22.7 (± 0.1) | 1.4 | 39.6 |
| BV173 | Cl | b2a2 | 23.7 (± 0.2) | 19.5 (± 0.5) | 4.2 | 6.3 |
| Patients without deletions | ||||||
| DS70 | Cl | b3a2 | 37.4 (± 0.2) | 24.9 (± 0.2) | 12.5 | 0.03 |
| JF69 | Co | b3a2 | 33.4 (± 0.03) | 27.9 (± 0.2) | 5.5 | 2.7 |
| JH55 | Cl | b2a2 | 30.6 (± 0.2) | 23.3 (± 0.02) | 7.3 | 0.9 |
| JK67 | Cl | b2a2 | 31.3 (± 0.1) | 24.7 (± 0.1) | 6.6 | 1.3 |
| KC61 | Cl | b2a2 | 30.8 (± 0.2) | 24.4 (± 0.06) | 6.4 | 1.5 |
| MA64 | Co | b3a2 | 35.9 (± 0.4) | 28.4 (± 0.4) | 7.5 | 0.7 |
| MT72 | Cl | b2a2 | 31.0 (± 0.3) | 24.3 (± 0.01) | 6.7 | 1.2 |
| RS89 | Cl | b2a2 | 34.0 (± 0.2) | 28.8 (± 0.2) | 5.2 | 3.5 |
| SA65 | Cl | b3a2 | 28.5 (± 0.2) | 21.6 (± 0.1) | 6.9 | 1.1 |
| SB60 | Cl | b3a2 | 30.4 (± 0.02) | 23.8 (± 0.04) | 6.6 | 1.3 |
| SG68 | Cl | b3a2 | 30.9 (± 0.2) | 24.3 (± 0.04) | 6.6 | 1.3 |
| TJ44 | Cl | b2a2 | 29.4 (± 0.1) | 24.1 (± 0.04) | 5.3 | 3.0 |
| Mean for patients without deletions | 6.9 | 1.1 | ||||
| Median for patients without deletions | 6.6 (5.7-7.2)* | 1.3 (2.4-0.9)* |
| Patient/cell line . | Translocation type . | Transcript type . | BCR-ABLCT . | B2MCT . | ΔCT . | BCR-ABLtranscripts, % of control gene . |
|---|---|---|---|---|---|---|
| Deleted cell line | ||||||
| MC3 | Cl | b3a2 | 24.5 (± 0.1) | 20.5 (± 0.01) | 4 | 6.9 |
| Patients with deletions | ||||||
| DS63 | Cl | b3a2 | 34.5 (± 0.5) | 27.0 (± 0.3) | 7.5 | 0.7 |
| JD66 | Co | b2a2 | 28.3 (± 0.1) | 22.2 (± 0.04) | 6.1 | 1.9 |
| MF68 | Cl | b3a2 | 38.5 (± 0.7) | 30.6 (± 0.1) | 7.9 | 0.5 |
| SP68 | Cl | b2a2 | 29.2 (± 0.2) | 24.7 (± 0.2) | 4.5 | 5.2 |
| Mean for patients with deletions | 6.5 | 1.4 | ||||
| Median for patients with deletions | 6.8 (4.9-7.8)* | 1.1 (4.0-0.6)* | ||||
| Nondeleted cell lines | ||||||
| MEG-01 | Cl | b2a2 | 24.1 (± 0.06) | 22.7 (± 0.1) | 1.4 | 39.6 |
| BV173 | Cl | b2a2 | 23.7 (± 0.2) | 19.5 (± 0.5) | 4.2 | 6.3 |
| Patients without deletions | ||||||
| DS70 | Cl | b3a2 | 37.4 (± 0.2) | 24.9 (± 0.2) | 12.5 | 0.03 |
| JF69 | Co | b3a2 | 33.4 (± 0.03) | 27.9 (± 0.2) | 5.5 | 2.7 |
| JH55 | Cl | b2a2 | 30.6 (± 0.2) | 23.3 (± 0.02) | 7.3 | 0.9 |
| JK67 | Cl | b2a2 | 31.3 (± 0.1) | 24.7 (± 0.1) | 6.6 | 1.3 |
| KC61 | Cl | b2a2 | 30.8 (± 0.2) | 24.4 (± 0.06) | 6.4 | 1.5 |
| MA64 | Co | b3a2 | 35.9 (± 0.4) | 28.4 (± 0.4) | 7.5 | 0.7 |
| MT72 | Cl | b2a2 | 31.0 (± 0.3) | 24.3 (± 0.01) | 6.7 | 1.2 |
| RS89 | Cl | b2a2 | 34.0 (± 0.2) | 28.8 (± 0.2) | 5.2 | 3.5 |
| SA65 | Cl | b3a2 | 28.5 (± 0.2) | 21.6 (± 0.1) | 6.9 | 1.1 |
| SB60 | Cl | b3a2 | 30.4 (± 0.02) | 23.8 (± 0.04) | 6.6 | 1.3 |
| SG68 | Cl | b3a2 | 30.9 (± 0.2) | 24.3 (± 0.04) | 6.6 | 1.3 |
| TJ44 | Cl | b2a2 | 29.4 (± 0.1) | 24.1 (± 0.04) | 5.3 | 3.0 |
| Mean for patients without deletions | 6.9 | 1.1 | ||||
| Median for patients without deletions | 6.6 (5.7-7.2)* | 1.3 (2.4-0.9)* |
The cycle threshold (CT) for BCR-ABL and the control gene B2M are shown. The ΔCT value for a sample corrects for any differences in complementary DNA between samples, and these values are directly comparable between samples. The mean, median, and interquartile range are shown for the ΔCT values of patients with and without deletions. The fold differences between the control gene andBCR-ABL were calculated from the equation, fold difference = (1 + E)−ΔCT, where E represents the efficiency of the PCR reaction. The fold differences are represented by BCR-ABL as a percentage of expression of the control gene. There were no differences in levels of transcripts between patients with deletions and those who lacked them by Mann-Whitney U test (P = 1.00).
CT indicates cycle threshold; Cl, classical Philadelphia translocation; Co, complex/variant translocation.
Interquartile range.