FACS analysis of hematopoietic tissues from rescuedscl−/− mice
| Tissue . | Surface marker . | Control YAC+scl+/− . | Rescue YAC+scl−/− . |
|---|---|---|---|
| Bone marrow | c-kit | 15.0 ± 2.44-150 | 12.8 ± 1.14-150 |
| CD34 | 6.7 ± 1.8 | 6.1 ± 0.9 | |
| Mac-1 | 30.8 ± 10.3 | 35.2 ± 3.6 | |
| Gr-1 | 33.0 ± 12.1 | 35.0 ± 2.6 | |
| Ter119 | 51.2 ± 10.2 | 42.9 ± 9.0 | |
| CD61 | 32.9 ± 11.5 | 36.1 ± 1.7 | |
| IgE/αIgE | 0.8 ± 0.34-150 | 0.9 ± 0.04-150 | |
| Spleen | B220 | 25.4 ± 10.7 | 23.2 ± 10.7 |
| CD4 | 5.2 ± 0.6 | 4.4 ± 1.4 | |
| CD8 | 4.6 ± 1.3 | 4.7 ± 1.3 | |
| Thymus | CD4−CD8− | 15.0 ± 8.6 | 12.6 ± 6.8 |
| CD4+CD8+ | 73.9 ± 7.4 | 76.1 ± 5.1 | |
| CD4+CD8− | 8.4 ± 2.6 | 8.9 ± 1.7 | |
| CD4−CD8+ | 2.8 ± 1.4 | 2.5 ± 0.2 | |
| Fetal liver | |||
| E11.5 | c-kit | 64.3 ± 18.9 | 72.0 ± 8.5 |
| E11.5 | Ter119 | 28.3 ± 24.8 | 20.5 ± 7.8 |
| E12.5 | c-kit | 1.7 ± 0.6 | 2.9 ± 0.9 |
| E12.5 | Ter119 | 85.0 ± 2.0 | 84.5 ± 2.9 |
| Tissue . | Surface marker . | Control YAC+scl+/− . | Rescue YAC+scl−/− . |
|---|---|---|---|
| Bone marrow | c-kit | 15.0 ± 2.44-150 | 12.8 ± 1.14-150 |
| CD34 | 6.7 ± 1.8 | 6.1 ± 0.9 | |
| Mac-1 | 30.8 ± 10.3 | 35.2 ± 3.6 | |
| Gr-1 | 33.0 ± 12.1 | 35.0 ± 2.6 | |
| Ter119 | 51.2 ± 10.2 | 42.9 ± 9.0 | |
| CD61 | 32.9 ± 11.5 | 36.1 ± 1.7 | |
| IgE/αIgE | 0.8 ± 0.34-150 | 0.9 ± 0.04-150 | |
| Spleen | B220 | 25.4 ± 10.7 | 23.2 ± 10.7 |
| CD4 | 5.2 ± 0.6 | 4.4 ± 1.4 | |
| CD8 | 4.6 ± 1.3 | 4.7 ± 1.3 | |
| Thymus | CD4−CD8− | 15.0 ± 8.6 | 12.6 ± 6.8 |
| CD4+CD8+ | 73.9 ± 7.4 | 76.1 ± 5.1 | |
| CD4+CD8− | 8.4 ± 2.6 | 8.9 ± 1.7 | |
| CD4−CD8+ | 2.8 ± 1.4 | 2.5 ± 0.2 | |
| Fetal liver | |||
| E11.5 | c-kit | 64.3 ± 18.9 | 72.0 ± 8.5 |
| E11.5 | Ter119 | 28.3 ± 24.8 | 20.5 ± 7.8 |
| E12.5 | c-kit | 1.7 ± 0.6 | 2.9 ± 0.9 |
| E12.5 | Ter119 | 85.0 ± 2.0 | 84.5 ± 2.9 |
Numbers indicate percentage of positive cells (± SD) following analysis of 4 adult mice or a minimum of 3 fetal livers for each genotype from line 2054.
Two animals analyzed.