Table 1. Integrity of human SCL/lacZ YAC... | American Society of Hematology

Table 1.

Integrity of human SCL/lacZ YAC within transgenic lines

Transgenic line	Transgene copy no.	Restriction fragments
Transgenic line	Transgene copy no.	Left arm	1	2	3	4	5	6	7	Right arm
2037	2.2	+	−	−	−	+	+	+	+	+a
2054	2.4	R	+	+	+	+	+	+	+	+
2074	2.3	R	+	+	+	+	+	+	+	+
2083	13.3	+	+	+	+	+	+	+b	+	+
2133	2.3	+	+	+	+	+	+	+	+	R

Transgenic line	Transgene copy no.	Restriction fragments
Transgenic line	Transgene copy no.	Left arm	1	2	3	4	5	6	7	Right arm
2037	2.2	+	−	−	−	+	+	+	+	+a
2054	2.4	R	+	+	+	+	+	+	+	+
2074	2.3	R	+	+	+	+	+	+	+	+
2083	13.3	+	+	+	+	+	+	+b	+	+
2133	2.3	+	+	+	+	+	+	+	+	R

Southern analysis was performed to assess the copy number and integrity of YAC transgenes. The integrity of 7 restriction fragments (1-7, Figure 1) was assessed, together with two additional fragments containing the left and right YAC arms.

+ indicates fragment of correct size detected; −, fragment absent; a, weak signal indicating deletion of the arm from one of the two YACs present in this line; b, two fragments detected, one of the correct size (10 copies) and one of a larger size (3 copies); R, fragment rearranged.

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