Overview of studies of bcl-2 rearrangement in persons without FL
| Author/region . | PCR method (no. cycles) . | Amount DNA . | Tissue samples . | Median age, y (range) . | Percentage positive . | Frequency (per 106 cells) . | Comments . |
|---|---|---|---|---|---|---|---|
| Limpens et al/Europe52 | Nested (50) | 1 μg | Follicular hyperplasia | 33 (7-78) | 56 (9/16) | 1-100 | — |
| — | — | Reactive tonsils | 4-8 | 50 (4/8) | 1-1000 | — | |
| — | — | Reactive nodes | NR | 0 (0/19) | NR | — | |
| — | — | Normal marrow | NR | 0 (0/30) | NR | — | |
| Aster et al/United States53 | Seminested (60) | 2 μg | Tonsils | 6.5 (2-32) | 25 (3/12) | 0.4-2.4 | — |
| — | — | Follicular hyperplasia | 34 (25-64) | 67 (2/3) | 0.5-4.6 | — | |
| Aster et al/Japan53 | — | — | Tonsils | 6 (3-40) | 50 (5/10) | 0.3-60.2 | — |
| Ohshima et al/Japan60 | Single-step (NR) | NR | Reactive nodes | NR | 11 (2/18) | NR | — |
| Liu et al/United States59 | Nested (60) | 2 μg | Sorted PB B cells | 50 (0-75) | 55 (29/53) | 0.8-32 | Frequency ∝ age |
| — | — | Autopsy spleen | 50 (0-85) | 35 (11/31) | 2.7-853 | Liver + 1/10; other tissues negative | |
| Bell et al/United States54 | Nested (60) | 2 μg | Sorted PB B-cells | Younger than 52 | 48 (59/122) | NR | ↑ In smokers, no association with age |
| Limpens et al/Europe58 | Seminested (56) | 1 μg | Sorted PB B-cells | NR | 67 (6/9) | ≈ 10 | — |
| Ji et al/United States57 | Seminested (46) | ≥ 7 μg | Normal PB | 6-60 and older | 63 (79/125) | 0.2-180 | Higher with age, M > F |
| Dolken et al/Europe55 | Nested (60) | 0.01-1 μg | PB mononuclear cells | 38 (23-90) | 46 (26/57) | 1-100 | No association with age |
| Fuscoe et al/United States56 | Nested (60) | 2.5 μg | PB lymphocytes | 27 (17-48) | 88 (30/34) | 0.08-960 | No association with age. M = F |
| Rauzy et al/Europe61 | Seminested (70) | ∼ 0.5 μg | Marrow | 68 (1-89) | 23 (11/48) | NR | Association with smoking |
| — | — | PB | 70 (1-100) | 44 (60/137) | NR | No association with age, sex, or smoking | |
| Delage et al/Canada63 | Nested (25 + 35) | 1 μg | PB mononuclear cells | 41 (38-64) | 100 (6/6) | 1 K-10 K | Multiple MBR, mcr in 5/6 |
| Summers et al/United Kingdom62 | RT-PCR (40) | 1.5 μg | PB | 42 (21-69) | 23 (112/481) | 104 | No association with age |
| Yasukawa et al65 | Nested (30) | 10 μg | PB mononuclear cells | NR | 44 (17/39) | NR | German population |
| — | — | PB mononuclear cells | NR | 15 (6/39) | NR | Japanese population | |
| Paltiel et al/Israel64 | Nested (NR) | NR | Sorted PB B cells | NR | 6.9 (24/346) | NR | No association with age, sex, smoking, or rural residence |
| Author/region . | PCR method (no. cycles) . | Amount DNA . | Tissue samples . | Median age, y (range) . | Percentage positive . | Frequency (per 106 cells) . | Comments . |
|---|---|---|---|---|---|---|---|
| Limpens et al/Europe52 | Nested (50) | 1 μg | Follicular hyperplasia | 33 (7-78) | 56 (9/16) | 1-100 | — |
| — | — | Reactive tonsils | 4-8 | 50 (4/8) | 1-1000 | — | |
| — | — | Reactive nodes | NR | 0 (0/19) | NR | — | |
| — | — | Normal marrow | NR | 0 (0/30) | NR | — | |
| Aster et al/United States53 | Seminested (60) | 2 μg | Tonsils | 6.5 (2-32) | 25 (3/12) | 0.4-2.4 | — |
| — | — | Follicular hyperplasia | 34 (25-64) | 67 (2/3) | 0.5-4.6 | — | |
| Aster et al/Japan53 | — | — | Tonsils | 6 (3-40) | 50 (5/10) | 0.3-60.2 | — |
| Ohshima et al/Japan60 | Single-step (NR) | NR | Reactive nodes | NR | 11 (2/18) | NR | — |
| Liu et al/United States59 | Nested (60) | 2 μg | Sorted PB B cells | 50 (0-75) | 55 (29/53) | 0.8-32 | Frequency ∝ age |
| — | — | Autopsy spleen | 50 (0-85) | 35 (11/31) | 2.7-853 | Liver + 1/10; other tissues negative | |
| Bell et al/United States54 | Nested (60) | 2 μg | Sorted PB B-cells | Younger than 52 | 48 (59/122) | NR | ↑ In smokers, no association with age |
| Limpens et al/Europe58 | Seminested (56) | 1 μg | Sorted PB B-cells | NR | 67 (6/9) | ≈ 10 | — |
| Ji et al/United States57 | Seminested (46) | ≥ 7 μg | Normal PB | 6-60 and older | 63 (79/125) | 0.2-180 | Higher with age, M > F |
| Dolken et al/Europe55 | Nested (60) | 0.01-1 μg | PB mononuclear cells | 38 (23-90) | 46 (26/57) | 1-100 | No association with age |
| Fuscoe et al/United States56 | Nested (60) | 2.5 μg | PB lymphocytes | 27 (17-48) | 88 (30/34) | 0.08-960 | No association with age. M = F |
| Rauzy et al/Europe61 | Seminested (70) | ∼ 0.5 μg | Marrow | 68 (1-89) | 23 (11/48) | NR | Association with smoking |
| — | — | PB | 70 (1-100) | 44 (60/137) | NR | No association with age, sex, or smoking | |
| Delage et al/Canada63 | Nested (25 + 35) | 1 μg | PB mononuclear cells | 41 (38-64) | 100 (6/6) | 1 K-10 K | Multiple MBR, mcr in 5/6 |
| Summers et al/United Kingdom62 | RT-PCR (40) | 1.5 μg | PB | 42 (21-69) | 23 (112/481) | 104 | No association with age |
| Yasukawa et al65 | Nested (30) | 10 μg | PB mononuclear cells | NR | 44 (17/39) | NR | German population |
| — | — | PB mononuclear cells | NR | 15 (6/39) | NR | Japanese population | |
| Paltiel et al/Israel64 | Nested (NR) | NR | Sorted PB B cells | NR | 6.9 (24/346) | NR | No association with age, sex, smoking, or rural residence |
NR indicates not recorded; PB, peripheral blood.