Engraftment characteristics of patient samples in NOD/SCID mice
| Patient . | Cells inoculated (× 106) . | Mice engrafted . | Rate of engraftment (d ± SD) . | Time of death (d) . | Level of engraftment at death (% human CD45+ cells ± SD) . | ||
|---|---|---|---|---|---|---|---|
| Bone marrow . | Spleen . | Peripheral blood . | |||||
| 1 | 5.0 | 2/2 | 133 ± 31 | 173 | 26.2 ± 5.0 | 33.5 ± 13.6 | 4.6 ± 3.6 |
| 2 | 2.5 | 4/4 | 53 ± 5 | 107 | 97.6 ± 2.0 | 95.5 ± 3.2 | 68.3 ± 26.8 |
| 3 | 3.5 | 4/4 | 25 ± 4 | 72 | 99.4 ± 0.3 | 94.8 ± 5.2 | 69.8 ± 7.1 |
| 4 | 3.0 | 4/4 | 57 ± 5 | 107 | 94.3 ± 1.5 | 91.9 ± 3.3 | 76.2 ± 0.4 |
| 5 | 5.0 | 3/3 | 164 ± 12 | 167 | 88.6 ± 2.7 | 27.1 ± 29.7 | 1.7 ± 1.1 |
| 6d | 3.9 | 1/2 | 84 | 123 | 97.1 | 81.4 | 93.5 |
| 6r | 6.6 | 2/2 | 77 ± 4 | 117 | 87.7 ± 16.6 | 66.1 ± 10.0 | 32.4 ± 12.5 |
| 7 | 4.9 | 2/2 | 19 ± 5 | 75 | 99.9 | 99.6 | 90.4 ± 1.5 |
| 8 | 3.5 | 2/2 | 47 ± 4 | 98 | 97.9 ± 1.3 | 99.2 ± 1.0 | 72.7 ± 16.1 |
| 9 | 7.9 | 1/3 | 121 | 183 | 97.9 | 99.0 | 84.9 |
| 10 | 3.8 | 3/3 | 31 ± 10 | 68 | 97.9 ± 1.6 | 92.9 ± 1.7 | 51.3 ± 11.0 |
| 11 | 9.9 | 3/3 | 45 ± 8 | 116 | 83.6 ± 15.6 | 97.5 ± 1.2 | 72.6 ± 7.5 |
| 12 | 3.9 | 1/1 | 168 | 136 | 29.5 | 75.1 | 1.0 |
| 13 | 7.6 | 2/3 | 190* | 196 | 83.7 ± 19.1 | 2.1 ± 2.3 | 2.1 ± 2.9 |
| 14 | 4.6 | 3/3 | 89 ± 28 | 142 | 18.0 ± 15.1 | 74.3 ± 30.6 | 33.4 ± 38.0 |
| 15 | 7.5 | 1/2 | 182 | 180 | 78.7 | 1.7 | 1.0 |
| 16 | 8.0 | 3/3 | 55 ± 11 | 102 | 99.2 ± 0.5 | 97.7 ± 0.9 | 49.1 ± 11.9 |
| 17 | 4.0 | 3/3 | 64 ± 7 | 95 | 99.6 ± 0.3 | 99.0 ± 0.6 | 85.4 ± 14.2 |
| 18 | 6.1 | 3/3 | 108 ± 12 | 176 | 94.4 ± 1.8 | 99.7 ± 0.1 | 67.4 ± 17.8 |
| 19 | 4.7 | 2/2 | 29 ± 11 | 66 | 98.2 ± 0.1 | 85.2 ± 3.6 | 51.5 ± 33.2 |
| Patient . | Cells inoculated (× 106) . | Mice engrafted . | Rate of engraftment (d ± SD) . | Time of death (d) . | Level of engraftment at death (% human CD45+ cells ± SD) . | ||
|---|---|---|---|---|---|---|---|
| Bone marrow . | Spleen . | Peripheral blood . | |||||
| 1 | 5.0 | 2/2 | 133 ± 31 | 173 | 26.2 ± 5.0 | 33.5 ± 13.6 | 4.6 ± 3.6 |
| 2 | 2.5 | 4/4 | 53 ± 5 | 107 | 97.6 ± 2.0 | 95.5 ± 3.2 | 68.3 ± 26.8 |
| 3 | 3.5 | 4/4 | 25 ± 4 | 72 | 99.4 ± 0.3 | 94.8 ± 5.2 | 69.8 ± 7.1 |
| 4 | 3.0 | 4/4 | 57 ± 5 | 107 | 94.3 ± 1.5 | 91.9 ± 3.3 | 76.2 ± 0.4 |
| 5 | 5.0 | 3/3 | 164 ± 12 | 167 | 88.6 ± 2.7 | 27.1 ± 29.7 | 1.7 ± 1.1 |
| 6d | 3.9 | 1/2 | 84 | 123 | 97.1 | 81.4 | 93.5 |
| 6r | 6.6 | 2/2 | 77 ± 4 | 117 | 87.7 ± 16.6 | 66.1 ± 10.0 | 32.4 ± 12.5 |
| 7 | 4.9 | 2/2 | 19 ± 5 | 75 | 99.9 | 99.6 | 90.4 ± 1.5 |
| 8 | 3.5 | 2/2 | 47 ± 4 | 98 | 97.9 ± 1.3 | 99.2 ± 1.0 | 72.7 ± 16.1 |
| 9 | 7.9 | 1/3 | 121 | 183 | 97.9 | 99.0 | 84.9 |
| 10 | 3.8 | 3/3 | 31 ± 10 | 68 | 97.9 ± 1.6 | 92.9 ± 1.7 | 51.3 ± 11.0 |
| 11 | 9.9 | 3/3 | 45 ± 8 | 116 | 83.6 ± 15.6 | 97.5 ± 1.2 | 72.6 ± 7.5 |
| 12 | 3.9 | 1/1 | 168 | 136 | 29.5 | 75.1 | 1.0 |
| 13 | 7.6 | 2/3 | 190* | 196 | 83.7 ± 19.1 | 2.1 ± 2.3 | 2.1 ± 2.9 |
| 14 | 4.6 | 3/3 | 89 ± 28 | 142 | 18.0 ± 15.1 | 74.3 ± 30.6 | 33.4 ± 38.0 |
| 15 | 7.5 | 1/2 | 182 | 180 | 78.7 | 1.7 | 1.0 |
| 16 | 8.0 | 3/3 | 55 ± 11 | 102 | 99.2 ± 0.5 | 97.7 ± 0.9 | 49.1 ± 11.9 |
| 17 | 4.0 | 3/3 | 64 ± 7 | 95 | 99.6 ± 0.3 | 99.0 ± 0.6 | 85.4 ± 14.2 |
| 18 | 6.1 | 3/3 | 108 ± 12 | 176 | 94.4 ± 1.8 | 99.7 ± 0.1 | 67.4 ± 17.8 |
| 19 | 4.7 | 2/2 | 29 ± 11 | 66 | 98.2 ± 0.1 | 85.2 ± 3.6 | 51.5 ± 33.2 |
A single mouse reached 1% human CD45+ cells in peripheral blood.