Effect of exogenous interleukin 2 on the susceptibility of CD4+ cells to apoptosis detected by annexin V staining
| Time of culture . | Annexin V–positive CD4+ cells (%) by time of culture . | ||
|---|---|---|---|
| 24 h . | 48 h . | 72 h . | |
| Control | |||
| No antigen | 6 | 2 | 2 |
| Anti-CD3 | 2 | 10 | 6 |
| Anti-CD3/IL-2 | 2 | 8 | 6 |
| Patient 1 | |||
| No antigen | 10 | 5 | 11 |
| Anti-CD3 | 22 | 20 | 21 |
| Anti-CD3/IL-2 | 24 | 16 | 20 |
| Patient 2 | |||
| No antigen | 2 | 3 | nd |
| Anti-CD3 | 2 | 9 | nd |
| Anti-CD3/IL-2 | 3 | 10 | nd |
| Patient 3 | |||
| No antigen | 2 | 4 | nd |
| Anti-CD3 | 3 | 18 | nd |
| Anti-CD3/IL-2 | 8 | 25 | nd |
| Time of culture . | Annexin V–positive CD4+ cells (%) by time of culture . | ||
|---|---|---|---|
| 24 h . | 48 h . | 72 h . | |
| Control | |||
| No antigen | 6 | 2 | 2 |
| Anti-CD3 | 2 | 10 | 6 |
| Anti-CD3/IL-2 | 2 | 8 | 6 |
| Patient 1 | |||
| No antigen | 10 | 5 | 11 |
| Anti-CD3 | 22 | 20 | 21 |
| Anti-CD3/IL-2 | 24 | 16 | 20 |
| Patient 2 | |||
| No antigen | 2 | 3 | nd |
| Anti-CD3 | 2 | 9 | nd |
| Anti-CD3/IL-2 | 3 | 10 | nd |
| Patient 3 | |||
| No antigen | 2 | 4 | nd |
| Anti-CD3 | 3 | 18 | nd |
| Anti-CD3/IL-2 | 8 | 25 | nd |
Patient 1: 12-year-old girl, 1 month after MUD-BMT; patient 2: 18-year-old girl, 1 month after CTX; patient 3: 6-month-old boy, 1 month after HD-CTX/ASCR. PBMCs were cultured and analyzed by FACS as described. Results are shown for interleukin 2 added to stimulated cultures at 25 U/mL.
nd indicates not done. See Table 1 for other abbreviations.