Granulocyte-macrophage colony-stimulating factor messenger RNA decay in eosinophils transduced with MEK1(E) or MKK3b(E)
| . | Half-life time ± SEM (min) . | t test . |
|---|---|---|
| Control | 12 ± 2 | P < .048 |
| TATMEK1(E) | 27 ± 7.3 | n = 5 |
| Control | 13 ± 5.2 | P = .820 |
| TATMKK3b(E) | 12 ± 2.6 | n = 3 |
| . | Half-life time ± SEM (min) . | t test . |
|---|---|---|
| Control | 12 ± 2 | P < .048 |
| TATMEK1(E) | 27 ± 7.3 | n = 5 |
| Control | 13 ± 5.2 | P = .820 |
| TATMKK3b(E) | 12 ± 2.6 | n = 3 |
Eosinophils were transduced for 2 hours with MEK1(E) or MKK3b(E) and then transfected with granulocyte-macrophage colony-stimulating factor (GM-CSF) messenger RNA. At different times after the transfection (from 0 to 60 minutes), the cells were harvested and total RNA was extracted. GM-CSF messenger RNA was quantified by Northern blot, normalized with β-Actin, and plotted versus time to determine for each condition the half-life time as presented. The statistical significance was analyzed by the Student t test.