Table 1. DNA strand break processing in... | American Society of Hematology

Table 1.

DNA strand break processing in CD34⁺ and CD34⁻ cells isolated from BM or CB after pulse exposure to EtNU

Sample no.	Source	Repair-induced SSB_t0			Time of 50% repair (t₅₀), min			Relative repair ratio (X × Y)
Sample no.	Source	CD34⁺	CD34⁻	Ratio CD34⁺/CD34⁻(X)	CD34⁺	CD34⁻	Ratio CD34⁺/CD34⁻ (Y)	Relative repair ratio (X × Y)
2	BM	143.3	53	2.7	105	20	5.3	14.2
3	BM	133.2	116.9	1.1	115	30	3.8	4.4
4	BM	258.6	140.2	1.8	130	70	1.9	3.4
5	BM	320.8	244.6	1.3	135	45	3	3.9
6	BM	105.2	14.4	7.3	50	25	2	14.6
8	BM	179.7	112	1.6	95	25	3.8	6.1
10	BM	196	73	2.7	25	15	1.6	4.3
11	BM	134.8	71.5	1.9	70	22	3	6
12	BM	207	125.9	1.6	45	30	1.5	2.5
13	BM	258.9	110.2	2.3	25	55	0.5	1.2
14	BM	182.3	93.9	1.9	60	30	2	3.9
17	BM	285.2	112.2	2.5	60	15	4	10.2
Mean BM				2.4			2.7	6.5
15	CB	142	55	2.6	40	18	2.1	5.4
16	CB	377.4	203.3	1.9	70	31	2.1	3.9
18	CB	142	126.7	1.1	40	12.5	3.2	3.6
19	CB	119.4	67.3	1.8	120	15	8	14.2
20	CB	248.7	124.5	2	30	20	1.5	3
1	CB	89.9	4.3	20.9^*	30	20	1.5	31.3^*
Mean CB				1.9			3.1	5.9
Total mean				2.2			2.8	6.2

Sample no.	Source	Repair-induced SSB_t0			Time of 50% repair (t₅₀), min			Relative repair ratio (X × Y)
Sample no.	Source	CD34⁺	CD34⁻	Ratio CD34⁺/CD34⁻(X)	CD34⁺	CD34⁻	Ratio CD34⁺/CD34⁻ (Y)	Relative repair ratio (X × Y)
2	BM	143.3	53	2.7	105	20	5.3	14.2
3	BM	133.2	116.9	1.1	115	30	3.8	4.4
4	BM	258.6	140.2	1.8	130	70	1.9	3.4
5	BM	320.8	244.6	1.3	135	45	3	3.9
6	BM	105.2	14.4	7.3	50	25	2	14.6
8	BM	179.7	112	1.6	95	25	3.8	6.1
10	BM	196	73	2.7	25	15	1.6	4.3
11	BM	134.8	71.5	1.9	70	22	3	6
12	BM	207	125.9	1.6	45	30	1.5	2.5
13	BM	258.9	110.2	2.3	25	55	0.5	1.2
14	BM	182.3	93.9	1.9	60	30	2	3.9
17	BM	285.2	112.2	2.5	60	15	4	10.2
Mean BM				2.4			2.7	6.5
15	CB	142	55	2.6	40	18	2.1	5.4
16	CB	377.4	203.3	1.9	70	31	2.1	3.9
18	CB	142	126.7	1.1	40	12.5	3.2	3.6
19	CB	119.4	67.3	1.8	120	15	8	14.2
20	CB	248.7	124.5	2	30	20	1.5	3
1	CB	89.9	4.3	20.9^*	30	20	1.5	31.3^*
Mean CB				1.9			3.1	5.9
Total mean				2.2			2.8	6.2

Cells were exposed to EtNU (100 μg/mL) for 20 minutes.

Values were excluded from mean.