Biochemical characterization of recombinant G6PD Amsterdam
| . | G6PD B . | G6PD Amsterdam . |
|---|---|---|
| Km G6P, μM | 64 ± 4 | 55 ± 5 |
| KmNADP, μM | 11 ± 1 | 11 ± 1 |
| Thermostability | Normal | Decreased |
| % utilization dG6P | 4 | 7.5 |
| % utilization GalP | 8 | 17 |
| % utilization 2-deoxy-NADP | 59 | 62 |
| % electrophoretic mobility | 100 | 110 |
| Specific activity, IU/mg | 210 ± 20 | 95 ± 3 |
| Ki NADPH, μM | 15 ± 2 | 14 ± 3 |
| . | G6PD B . | G6PD Amsterdam . |
|---|---|---|
| Km G6P, μM | 64 ± 4 | 55 ± 5 |
| KmNADP, μM | 11 ± 1 | 11 ± 1 |
| Thermostability | Normal | Decreased |
| % utilization dG6P | 4 | 7.5 |
| % utilization GalP | 8 | 17 |
| % utilization 2-deoxy-NADP | 59 | 62 |
| % electrophoretic mobility | 100 | 110 |
| Specific activity, IU/mg | 210 ± 20 | 95 ± 3 |
| Ki NADPH, μM | 15 ± 2 | 14 ± 3 |
Values are means ± SD of 3 independent measurements.