Clone sources and description of myeloid complementary DNA microarray
| Sources . | No. clones . | Description . |
|---|---|---|
| LRH clones: Flow-sorted HSCs from mouse bone marrow | 1288 | Subtracted myeloid cDNA library; represent early myeloid stem/progenitor cells |
| EEE clones: EML cells and differentiated derivatives | 310 | Subtracted myeloid cDNA library, composed of EML(0), EML(6) and EPRO cDNA libraries; represent late-stage myeloid cells; and clones derived from RDA |
| cDNAs from assorted mouse tissues (Research Genetics) | 692 | Assortment of regulatory and housekeeping genes expressed in a variety of cell types |
| Putative Evi-1 target genes | 96 | cDNAs identified by in vitro selection for Evi-1-binding sites |
| T-cell RDA clones8-150 | 576 | T cell–specific genes |
| Total | 2962 |
| Sources . | No. clones . | Description . |
|---|---|---|
| LRH clones: Flow-sorted HSCs from mouse bone marrow | 1288 | Subtracted myeloid cDNA library; represent early myeloid stem/progenitor cells |
| EEE clones: EML cells and differentiated derivatives | 310 | Subtracted myeloid cDNA library, composed of EML(0), EML(6) and EPRO cDNA libraries; represent late-stage myeloid cells; and clones derived from RDA |
| cDNAs from assorted mouse tissues (Research Genetics) | 692 | Assortment of regulatory and housekeeping genes expressed in a variety of cell types |
| Putative Evi-1 target genes | 96 | cDNAs identified by in vitro selection for Evi-1-binding sites |
| T-cell RDA clones8-150 | 576 | T cell–specific genes |
| Total | 2962 |
HSCs indicate hematopoietic stem cells; RDA, representation difference analysis.
From B. Lu, S. Kim, and R. A. Flavell, unpublished data, 2001.