Modulation of T-cell proliferation by monoclonal antibodies neutralizing BMSC-derived cytokines
| MLRs* . | Stimulation index (mean ± SD) . |
|---|---|
| T cells + PBLs | 60 ± 5 |
| T cells + PBLs + BMSCs | 25 ± 3† |
| T cells + PBLs + BMSCs + anti-rhIL-6 (5 μg/mL) | 24 ± 4‡ |
| T cells + PBLs + rhIL-6 (5 μg/mL) | 55 ± 32-153 |
| T cells + PBLs + BMSCs + anti-rhIL-11 (50 μg/mL) | 20 ± 2‡ |
| T cells + PBLs + rhIL-11 (50 μg/mL) | 52 ± 22-153 |
| T cells + PBLs + BMSCs + anti-rhTGF-β (0.1 μg/mL) | 25 ± 2‡ |
| T cells + PBLs + BMSCs + anti-rhTGF-β (1 μg/mL) | 45 ± 12-153 |
| T cells + PBLs + rhTGF-β (1 μg/mL) | 35 ± 8† |
| T cells + PBLs + BMSCs + anti-rhHGF (0.25 μg/mL) | 24 ± 3‡ |
| T cells + PBLs + BMSCs + anti-rhHGF (2.5 μg/mL) | 39 ± 82-153 |
| T cells + PBLs + rhHGF (2.5 μg/mL) | 38 ± 5† |
| T cells + PBLs + BMSCs + anti-rhTGF-β (1 μg/mL) + anti-rhHGF (2.5 μg/mL) | 55 ± 22-153 |
| T cells + PBLs + rhTGF-β (1 μg/mL) + rhHGF (2.5 μg/mL) | 27 ± 3† |
| MLRs* . | Stimulation index (mean ± SD) . |
|---|---|
| T cells + PBLs | 60 ± 5 |
| T cells + PBLs + BMSCs | 25 ± 3† |
| T cells + PBLs + BMSCs + anti-rhIL-6 (5 μg/mL) | 24 ± 4‡ |
| T cells + PBLs + rhIL-6 (5 μg/mL) | 55 ± 32-153 |
| T cells + PBLs + BMSCs + anti-rhIL-11 (50 μg/mL) | 20 ± 2‡ |
| T cells + PBLs + rhIL-11 (50 μg/mL) | 52 ± 22-153 |
| T cells + PBLs + BMSCs + anti-rhTGF-β (0.1 μg/mL) | 25 ± 2‡ |
| T cells + PBLs + BMSCs + anti-rhTGF-β (1 μg/mL) | 45 ± 12-153 |
| T cells + PBLs + rhTGF-β (1 μg/mL) | 35 ± 8† |
| T cells + PBLs + BMSCs + anti-rhHGF (0.25 μg/mL) | 24 ± 3‡ |
| T cells + PBLs + BMSCs + anti-rhHGF (2.5 μg/mL) | 39 ± 82-153 |
| T cells + PBLs + rhHGF (2.5 μg/mL) | 38 ± 5† |
| T cells + PBLs + BMSCs + anti-rhTGF-β (1 μg/mL) + anti-rhHGF (2.5 μg/mL) | 55 ± 22-153 |
| T cells + PBLs + rhTGF-β (1 μg/mL) + rhHGF (2.5 μg/mL) | 27 ± 3† |
BMSC, bone marrow stromal cell; MLR, mixed lymphocyte reaction; PBL, peripheral blood lymphocyte; rhIL-6, recombinant human interleukin 6; TGF, transforming growth factor; hepatocyte growth factor.
CD2+ (5 × 104) T cells were mixed at 1:1 ratio with allogeneic PBLs ± BMSCs.
Statistically different as compared with MLRs without BMSCs (P = .0001).
Not statistically different as compared with MLRs with BMSCs (P = .05).
Not statistically different as compared with MLRs without BMSCs (P = .05).