Specific antibody-binding capacity (SABC) of TPA-treated and -untreated stably transfected K562 clones
| MAb . | Directed against . | K562/Lu (control) . | K562/RhD . | K562/RhAG . | K562/RhD/RhAG . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TPA . | TPA . | TPA . | TPA . | ||||||||||
| 0 h . | 24 h . | 48 h . | 0 . | 24 h . | 48 h . | 0 . | 24 h . | 48 h . | 0 . | 24 h . | 48 h . | ||
| 6H9 | CD47 | 41.9 | 32.8 | 30.0 | 45.8 | 26.2 | 26.6 | 81.7 | 69.7 | 88.9 | 47.4 | 36.2 | 37.9 |
| 2D10 | RhAG | 52.8 | 13.8 | 9.1 | 40.0 | 14.0 | 4.6 | 133.0 | 326.0 | 426.0 | 79.3 | 100.9 | 191.0 |
| BRIC69 | Rh | 1.9 | 0.5 | 0.0 | 7.8 | 1.2 | 0.0 | 4.8 | 1.8 | 1.5 | 25.2 | 16.8 | 23.8 |
| LM342 | Lub | 109.0 | 320.0 | 454.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| MAb . | Directed against . | K562/Lu (control) . | K562/RhD . | K562/RhAG . | K562/RhD/RhAG . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TPA . | TPA . | TPA . | TPA . | ||||||||||
| 0 h . | 24 h . | 48 h . | 0 . | 24 h . | 48 h . | 0 . | 24 h . | 48 h . | 0 . | 24 h . | 48 h . | ||
| 6H9 | CD47 | 41.9 | 32.8 | 30.0 | 45.8 | 26.2 | 26.6 | 81.7 | 69.7 | 88.9 | 47.4 | 36.2 | 37.9 |
| 2D10 | RhAG | 52.8 | 13.8 | 9.1 | 40.0 | 14.0 | 4.6 | 133.0 | 326.0 | 426.0 | 79.3 | 100.9 | 191.0 |
| BRIC69 | Rh | 1.9 | 0.5 | 0.0 | 7.8 | 1.2 | 0.0 | 4.8 | 1.8 | 1.5 | 25.2 | 16.8 | 23.8 |
| LM342 | Lub | 109.0 | 320.0 | 454.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
SABC values (× 10−3) were determined by indirect immunofluorescence using QIFIKIT calibrated beads (see “Materials and methods”). The values represent an estimate of the mean number of antibody molecules bound per cell. TPA indicates 12-O-tetradecanoyl phorbol 13-acetate.